The Journal of American Science
(J Am Sci)
ISSN 1545-1003 (print); ISSN 2375-7264 (online),
Volume 11, Special Issue 2 (Supplement Issue 2),
April 25, 2015
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Titles / Authors
Diabetic Autonomic Neuropathy, a Co-factor related to
Recurrent Urinary Tract Infections
In Type2 Diabetic females, a Study in an Egyptian
El-Sheikh, 1 Nora A. El-Sheikh, 2
Manal M.Badawi, 2 Magda H. Mahran and 3
Nirvana A. Khalaf
Seifein Diabetes Center, Cairo, Egypt
& Immunology Department (RIO), Research Institute of
Ophthalmology, Giza, Egypt.
Pathology Unit (RIO), Research Institute of
Ophthalmology, Giza, Egypt.
Bacterial infection is a common finding in diabetics
presenting in outpatient clinics. It may occur in many
organs, although urinary tract infection (UTI) is the
most frequent. Women are significantly likely to develop
UTI then men (1). Diabetic Autonomic Neuropathy (DAN) is
a serious complication of diabetes (2). It is among the
least recognized and understood complications despite
its significant negative impact on survival (2,4).
Aim of Study: The aim of our study is to correlate
the occurrence of recurrent UTI with DAN in a female
diabetic type2 population. Materials & Methods:
We recruited 60 female patients suffering from recurrent
UTI, age range from 30 – 50 years, diagnosed as type2
diabetes since a period of less than 5 years with
average HbA1c of 7.5% range (7.2-8.1). All patients were
subject to SudoScan test to diagnose DAN, Clinical
examination and Laboratory assessment. Results:
patients were categorized into three groups: group A: 20
patients suffering from mild DAN (40-50-uS) and mild UTI
(pus cells 30-50 /HPF) - group B: 24 patients with
moderate DAN (25-39 uS) and moderate UTI (pus cells
51-80 HPF) and (group C: 12 patients with severe DAN (<
25 uS) and severe UTI (pus cells > 100 /HPF).
Culture & Sensitivity results demonstrated a variety of
micro-organisms mainly Escherichia coli,
Staphylococcus saprophyticus, Klebsiella spp.,
group B streptococcus and Candida albicans. A
combination of 2 to 3 antimicrobial agents was selected
for every patient to be used for about 1-2 weeks.
28cases - half from every group - were randomly selected
for adding anti-neuropathy agents. After 2 months, all
patients presented for a second urine analysis, culture
and HbA1c. The Results were as follow: 24 patients among
the 28 selected for anti-neuropathy treatment showed
negative results of culture & sensitivity compared to12
patients among the other 28 patients who received only
antibiotic therapy (P<0.005). HbA1c decreased on average
These results along with the matching results of the
Sudoscan prove the correlation between the presence of
DAN and UTI and highlight its role as a co-factor with
hyperglycemia in the pathogenesis of recurrent UTI.
El-Sheikh, Nora A. El-Sheikh, Manal M. Badawi, Magda H.
Mahran and Nirvana A. Khalaf. Diabetic Autonomic
Neuropathy, a Co-factor related to Recurrent Urinary
Tract Infections In Type2 Diabetic females, a
Study in an Egyptian Population.
J Am Sci
2015;11(2s):1-5]. (ISSN: 1545-1003).
Urinary Tract Infection in diabetics, Sudoscan, Type 2
Diabetes Mellitus, Diabetic Autonomic Neuropathy.
The effect of a training tool in Score level and some
physiological Parameters of butterfly swimmers
Wael Abdel Moati Khalafalla
Department of Swimming, Faculty of Physicsal Education,
El Azhar University. Egypt.
Abstract: Research aims:
1. knowing the changes in score
levels of butterfly swimmers before and after monofin
training program. 2. Knowing the changes in some
physiological parameters under investigation of
butterfly swimmers before and after monofin training
program. Research Methods: Experimental approach
was used, by experimental design of one group of
swimmers in a pre and post measurement. Participants:
After a full explanation of the aim of the study and of
the experimental procedures, 8 male swimmers from El
Azhar University in the year (2013-2014), Procedures:
Before experiments, swimmers were asked to abstain
from smoking or medicament and caffeine. They were
participating in training using Monofin at Saraya El
Kobba youth center in swimming pool. They started with
warming up for 10 minutes, trained using Monofin for 60
minutes at the end of the training they were practise
cooling down for 10 minutes. The training period was
between 1/10 – 31/12/2013 for 12 weeks, during the
training they were always motivated and encouraged to
finish their task. A the end of the training program,
100 meters butterfly swim was timed and the record level
was assessed and recording the time of each participant.
Blood samples were drawn at rest and after 100 meters
butterfly swim, before and after the 12 weeks training
course, together with B/P, pulse rate and Vo2
max, which was performed using the Ergometer and Astrand
Nomogram. Results: Hemoglobin, RBCs, hematocrit
revealed an increased levels after the training program,
which means that the Monofin training increased oxygen
capacity of the participants. The increased WBCs and
cortisol levels after the training period indicated a
better immunity and health change following Monofin
training, this result was also recorded in case of the
increased CD4, CD8 after Monofin training. CD8 occurs on
the surface of cytotoxic T cells, while CD4 occurs on he
surface of helper T cells. Both foster Lymphocite
development and immunity Vo2 max increased after monofin
training program and land exercise from 38.2 ± 1.9 to
45.4 ± 1.7 ml/kg/min. Conclusion: 1. Monofin
enhance swimmers speed, performance and score levels. 2.
Monofin led to positive results affecting VO2 max, pulse
rate, B/P. 3. The training program affect positively
Hemoglobin, RBCs, Hematocrit and increased oxygen
carriers. 4. The training program affect positively the
immunological variables and health state of the
Abdel Moati Khalafalla. The effect of a
training tool in Score level and some physiological
Parameters of butterfly swimmers.
J Am Sci
2015;11(2s):6-12]. (ISSN: 1545-1003).
effect; tool; Score; physiological Parameter; swimmer
Clinical Usefulness of
in Patients with
Ashraf Abd Eldayem 2 and Amal F. Gharib3
Departments of 1
Clinical Oncology & Nuclear medicine, 2
Orthopedic Surgery, 3 Medical Biochemistry,
Faculty of Medicine, Zagazig University, Egypt
This study was
designated to investigate whether telomerase is
reactivated in Ewing’s sarcoma and whether it has a
Patients and Methods:
Thirty-two patients with localized Ewing's sarcoma of
bone were treated with
four VACA blocks,
each block lasted
9 weeks and consisted of three courses of
treatment each, administered at 3-week intervals.
Local therapy was
scheduled to be given before the second block and was
individually planned for each patient.
activity was determined using a modified-TRAP assay,
hTERT mRNA Expression
was performed by quantitative real-time polymerase chain
reaction and the
telomere length was determined based on the telomere
restriction fragment (TRF) – Southern blot analysis.
patients (44%) had a complete response or very good
partial response (VGPR). Fifteen patients
(47%) had either a partial response or stable disease.
(TA) was detected in 84%
of patients. No significant correlation was found
between any of the clinical parameters and TA. High
human telomerase reverse transcriptase
(hTERT) (≥100 copies) was identified in 59% of patients
and it was significantly correlated with high TA.
Twenty-one of 32 patients exhibited changes in telomere
lengths: eight longer and thirteen shorter. No
significant correlation was found between telomere
length and TA.
Nineteen (83%) of 23 patients with high TA
relapsed, while none of the nine patients
with low TA did. Highly significant correlation was
observed between TA and progression free
survival (PFS). Low TA patients had 100% PFS,
while high-TA patients had 36% PFS (P < .0001).
patients with high and low hTERT expression
was statistically significant (P =
All samples that were
tested for hTERT were also analyzed for TA
and correlated significantly (P =.0.0357).
Our results showed that telomerase activity could be
used as a prognostic factor in patients with Ewing's
[Alaa Fayed, Ashraf Abd Eldayem
and Amal F. Gharib..
Usefulness of Telomerase Assay
in Patients with
Ewing's Sarcoma. J Am Sci
Ewing's sarcoma, chemotherapy, telomerase assay.
manuscripts in this issue are presented as online first for
peer-review, starting from May 29, 2015.
comments are welcome:
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