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Science Journal

 

The Journal of American Science

ISSN 1545-1003

Volume 6, Issue 11, Cumulated No. 32, November 1, 2010

Cover Page, Introduction, Contents, Call for Papers, am0611

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Welcome to send your manuscript(s) to: americansciencej@gmail.com.

CONTENTS  

  No.

Titles / Authors

page

 

1

Indirect Boundary Element Method for Calculation of Compressible Flow past a Symmetric Aerofoil with Linear Element Approach Using Doublet Distribution Alone

Muhammad Mushtaq*  &  Nawazish Ali Shah

Department of Mathematics, University of Engineering & Technology Lahore – 54890, Pakistan

Corresponding Author, e-mail:  mushtaqmalik2004@yahoo.co.uk

Abstract: In this paper, an indirect boundary element method is applied to calculate the compressible flow past a symmetric aerofoil. The velocity distribution for the flow over the surface of the symmetric aerofoil has been calculated with linear boundary element approach using doublet distribution alone. To check the accuracy of the method, the computed flow velocity is compared with the exact velocity. The comparison of these results has been given in the tables and graphs. It is found that the computed results are in good agreement with the analytical results. [Journal of American Science. 2010;6(11):1-9]. (ISSN: 1545-1003).

Keywords: Indirect boundary element method, Compressible flow, Velocity distribution, Symmetric aerofoil, linear element.

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Use of Long Yam Bean (Sphenostylis stenocarpa) as Soil Amendment for the Growth, Leaf Chemical Composition and Yield of White Yam (Dioscorea rotundata L)

Emmanuel Ibikunoluwa Moyin-Jesu1 and Francis Omotayo Adekayode2

1Agronomy Department, Federal College of Agriculture Akure, Nigeria

moyinjesu2004@yahoo.com

2Department of Crop, Soil and Pest Management, Federal University of Technology, Akure, Nigeria.

adekay98@yahoo.com

Abstract: An experiment was carried out to investigate the use of long yam bean (Sphenostilies sternocarpa) as soil amendment for the growth and yield of white yam (Dioscorea rotundata L) between 1999 and 2003 at Akure in the rain forest zone of Nigeria. There were four treatment namely; NPK 15 – 15 – 15 fertilizer applied at 250kg/ha, poultry manure at 6 t/ha, long yam beans planted at two seeds per hole at a spacing of 1m x 0.5m between rows of yam plots (soil amendment) and a control (no fertilizer). The treatments were arranged in a randomized complete block design (RCB) and replicated five times. The soil analyses before planting and after harvesting were carried out.  Each plot size is 4m x 4m (16m2).  The growth parameters measured for the yam were vine length (cm), leaf population and stem girth (cm).  At harvest, yam tuber weight (kg), tuber length (cm) tuber girth; root length and seed yield of long yam bean plants were determined. The leaf and soil N, P, K, Ca, Mg, pH and organic matter contents were also analysed at end of the experiment. The results showed that there were significant (p<0.05) increases in the vine length, leaf population, stem girth, tuber weight, tuber length, tuber girth, soil and leaf N, P, K, Ca, Mg; pH and organic matter of white yam cultivated under the different fertilizer treatments compared to the control treatment. Long yam bean plants used as soil amendment increased the yam vine length, stem girth, leaf population, tuber weight, tuber length and tuber girth by 81% 88.4%, 69.5%, 88.97%, 76% and 94% compared to the control.  The same treatment (long yam bean plants) also increased the leaf population, tuber weight, tuber length and tuber girth of yam by 11%, 31%, 30% and 55% respectively compared to NPK fertilizer treatment. Long yam plants also increased the soil pH, O.M, K, Ca and Mg by 29%, 92%, 97%, 86%, 96%, 97% and 89% respectively compared to the control treatment.  It increased soil pH, organic matter, K Ca and Mg by 31%, 87%, 1.42, 98% and 98.5% compared to NPK fertilizer. Long yam plants gave seed yield of 2.3 t/ha and produced yam tuber yield of 4900kg/ha amounting to $6,050 compared to $3453.00 and $3,380.00 estimated on yam yields alone under poultry manure and NPK fertilizer treatments. Finally, the use of long yam bean plants as biological fertilizer source for yam production could substitute for 250kg/ha NPK fertilizer and 6t/ha poultry manure. [Journal of American Science. 2010;6(11):10-17]. (ISSN: 1545-1003).

Keywords: Long yam bean, soil amendment, white yam performance

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Biochemical and Molecular Profiles of Gibberellic Acid Exposed Albino Rats

Hanan A.E.Soliman1; Mona M. Mantawy2 and Hany M. Hassan3

1Chemistry Departement, Biochemistry Branch, Faculty of Science, Beni suef University, Egypt. 2; Department of Medicinal chemistry , National Research Center, Dokki , Egypt and 3 Immunobiology and immunopharmacology unit , Animal Reproduction Research Inst., Giza , Egypt

Abstract: The present study casts the light on  the influence of the  plant growth regulator, Gibberellic acid (GA3), on antioxidant defense systems [glutathione peroxidase, superoxide dismutase (SOD), and catalase (CAT)], lipid peroxidation level (malondialdehyde = MDA), AST, ALT , alkaline phosphatase, creatinine, total protein , albumin globulin , total lipids , total cholesterol, calcium  and glucose . Moreover, histopathological examination of kidney and liver was done. On the molecular level, the DNA damage was determined. The rats were received 75 ppm of GA3 in drinking water   ad libitum for 50 days. Gibberellic acid (GA3) treatments caused different effects on the estimated parameters compared to control. Gibberellic acid exposure induced significant elevations of plasma AST, ALT, alkaline phosphatase, creatinine and malondialdehyde. However, Gibberellic acid produced non significant alterations in plasma total protein, albumin globulin, total lipids, total cholesterol, calcium and glucose. On the other hand, exposure elucidate significant reductions of catalase, superoxide dismutase and glutathione peroxidase in comparison to control group. The histopathological findings revealed that Kidney sections of Gibberellic acid treated rats suffered from areas of interstitial fibrosis which appear as segmental and global glomerular sclerosis tubulointerstitial injury. On the similar ground, liver section of Gibberellic acid treated rats revealed that Gibberellic acid induced liver    fibrosis; fatty metamorphosis and necrosis. The total genomic DNA electrophoretic pattern of lymphocytes deprived from  Gibberellic acid treated rats revealed strong and obvious DNA damage as represented by a lot of fragments migrated from the wells. As a conclusion, Gibberellic acid (75 ppm) produce hepatonephrotoxicity, subsequently has oxidative stress role and DNA damage in albino rats 50 days post treatment. [Journal of American Science. 2010;6(11):18-23]. (ISSN: 1545-1003).

Keywords: plant growth regulator; Gibberellic acid (GA3); antioxidant defense systems; superoxide dismutase (SOD); catalase (CAT); lipid peroxidation

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An Efficient Algorithm for Transforming XML Schema into Relational Schema

1 Abad Shah, 2Amjad Farooq, 3Syed Ahsan

1,3 Al-Khawarizmi Institute of Computer Science, University of Engineering and Technology, Lahore

2 Department of Computer Science, University of Engineering and Technology, Lahore

Amjadfarooquet@gmail.com

ABSTRACT: The Web and XML have influenced all walks of life especially those that involve business activities over the Internet. People like to do their business activities and transactions from their homes to save time and money. Many business and commercial companies such as insurance companies and banks maintain their records using relational database management systems. But the traditional relational database technology is unable to provide all these new facilities to the users. To enable the traditional relational database technology to cope with the new challenges of the Web and XML technologies, we need a transformation between the XML technology and the relational database technology as a middleware. To achieve this objective, we already proposed and reported an algorithm. In this paper, we extend our previous work and present automation details, testing, and performance report of our proposed algorithm. The result shows that the implementation of the algorithm is more efficient than the existing algorithms for the same purpose [Journal of American Science. 2010;6(11):24-37]. (ISSN: 1545-1003).

Keywords: XML, web, rational database, transforming algorithm

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Effect of injector types, irrigation and nitrogen levels on II-Garlic yield, water and nitrogen use efficiency

Tayel, M.Y.,*Shaaban, S.M., Ebtisam I. El-Dardiry and Sabreen Kh.

Water Relations and Field Irrigation Dept., National Research Centre, Dokki, Cairo, Egypt.

*shaabansm@yahoo.com

Abstract: Field experiments were conducted during two consecutive growing seasons in split split plot design on a clay loam soil at Shalaquan, Qalubia Governorate, Egypt. Experiments investigated the effect of three injectors types by-bass pressurized mixing  tank (J1), venturi (J2) and piston pump (J3); three rates of irrigation 50, 75; 100% of ETc (I1, I2; I3 ); three nitrogen levels 60, 90; 120 kg fed-1 (N1, N2; N3) on garlic yield, water use efficiency (WUE) and nitrogen use efficiency (NUE). The main results could be summarized as follows; the maximum and minimum garlic yields (6.34, 2.38 ton fed-1) were obtained with treatment J3 I2 N3 and J1 I1 N1, respectively. Maximum value of WUE was 3.29 kg garlic m-3 of irrigation water as recorded with the treatment J3 I1 N3, while the minimum value was 1.30 kg garlic m-3 of irrigation water as recorded with the treatment J1 I3 N1. The maximum and minimum values of NUE in kg garlic kg-1 N were 83.22 and 29.17 for J2 I2 N1 and J1 I1 N3, respectively. A positive linear relationship was found between WUE and NUE. [Journal of American Science. 2010;6(11):38-46]. (ISSN: 1545-1003).

Keywords: Field experiments; clay loam soil; water use efficiency (WUE); nitrogen use efficiency (NUE)

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Software Cost Estimation through Entity Relationship Model

Arshid Ali 1, Salman Qadri 2, Syed Shah Muhammad 2, Jalil Abbas 3, Muhammad TariqPervaiz 2,

Sarfaraz Awan 2

1. Department of Computer Science, GCU Faisalabad, Pakistan

2. Department of Computer Science, Virtual University of Pakistan, Lahore, Pakistan

3. Department of Computer Science, University of Central Punjab, Lahore, Pakistan

sayyed_qadri@hotmai.com

Abstract: Software Cost Estimation is essential for efficient control and management of the whole software development process. Today, Constructive Cost Model (COCOMO 11) is very popular for estimating software cost. In Constructive Cost Model lines of code and function, points are used to calculate the software size. Actually, this work represents the implementation stages but in early stages in software development, it was not easy to estimate software cost. The entity relationship model (ER Model) is very useful in requirement analysis for data concentrated systems. This paper highlights the use of Entity Relationship Model for software cost estimation. Pathway Density is ushered in. By using the Pathway Density and other factors, many regression models are built for estimating the software cost. So in this paper, Entity Relationship Model is based on estimated cost of software. [Journal of American Science. 2010;6(11):47-51]. (ISSN: 1545-1003).

Keywords: ER Model, Cost Estimation, Entity

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Web-Ontology Design Quality Metrics

                1Amjad Farooq, 2Syed Ahsan, 2Abad Shah

2 Al-khawarizmi Institute of Computer Science

1Department of Computer Science and Engineering

University of Engineering and Technology, Lahore

amjadfarooq@uet.edu.pk

Abstract: Semantic Web is an extension of current web in which the web resources are equipped with formal semantics about their interpretation for the machines.  These web resources are integrated in the form of web information systems, and their formal semantics are normally represented in the form of web-ontologies. Using the database terminology, we can say that web-ontology of a semantic web system is schema of that system. Since web-ontology is an integral element of semantic web systems, therefore, design quality of a semantic web system can be measured by measuring the quality of its web-ontology. The key consideration is that after completing design of a web-ontology, it is appropriate time to assess its quality so that in case, the design is of low quality, it can be improved before its instantiation. This can save a considerable amount of cost and effort for developing high quality semantic web systems. Metrics are considered as suitable tools for evaluating quality. In this paper, we propose certain metrics for web-ontology quality evaluation. These metrics may contribute in developing a high quality semantic web system. [Journal of American Science. 2010;6(11):52-58]. (ISSN: 1545-1003).

Keywords: Semantic web; Ontology metrics; quality measurement

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Determining Regression Models of Almond and its Kernel Mass Based on Geometric Properties (Shahrud 12 and Mama'e Varieties)

A.Mohamadi1, M.A.Ghazavi2, B.Hosseinzadeh2

1Payame-noor university, Farsan, Iran

2 Department of mechanical farm machinery,University of Shahrekord, Shahrekord 115 Iran

bahram_hs@yahoo.com

Abstract: Almond (Prunus amygdalus) belongs to Rosaceae family and sub-family of Pronoideae. Physical traits of agricultural products are main parameters in designing of grading, conveying, processing, and packing systems. In this study the physical traits such as dimensions, mass, volume, sphericity, geometric average of Mama'e and Shahrud 12 almonds and their kernels were measured and calculated. The average amounts of length, width, and thickness for both almond varieties were 37.41, 23.21, and 16.63 mm, respectively, and for almonds' kernel were 28.05, 13.4, and 7.82 mm, respectively. Results from modeling of almond and its kernel masses based on dimensions and volume showed that there exists a great correlation coefficient between the samples actual volumes and masses, but since determining actual volume of almond and its kernel is a time-taking task, it was suggested to use calculated volume and presuming that the cross-sectional area of the almond is oval. Also the mass model based on the thickness had the highest determination coefficient and lowest regression error which was the best option for industrial and economical applications. [Journal of American Science. 2010;6(11):59-64]. (ISSN: 1545-1003).

Keywords: Almonds, Physical Properties, Mass Modeling, Dimensional Models, Volumetric Models

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Regional assessment of groundwater vulnerability in Tamtsag basin, Mongolia using drastic model

Fanomezantsoa Hasiniaina1, Jianwei Zhou, Luo Guoyi

1School of Environmental Studies, China University of Geosciences (Wuhan)

Lumo Road 388, Wuhan City, 430074 Hubei Province, P.R. China.

hasiniainaf@hotmail.com

ABSTRACT: Groundwater is one of the most valuable natural resources and for that reason, its protection and management is vital for human evolution, socio-economic development and ecological diversity. Because of the known health and economic impacts associated with groundwater contamination, steps to assess groundwater vulnerability must be taken. This study aimed to assess groundwater pollution potentials of the north-eastern part of the deep confined aquifer of block XIX, Tamtsag basin, Mongolia. The normal DRASTIC model was applied to the study area with the help of GIS. DRASTIC parameters were calculated from geological data, soil and elevation contour maps, and groundwater level data of the study area. ArcInfo/GIS was used to demarcate vulnerable zones based on their vulnerability index. Finally, a sensitivity analysis of the parameters constituting the model was performed in order to evaluate the relative importance of the each DRASTIC model parameters. The aquifer vulnerability map revealed that only 2% of the study area is under moderate vulnerability to contamination, the remaining zone was determined to be in a low risk category. GIS greatly facilitated the implementation of the sensitivity analysis applied on the DRASTIC vulnerability index which otherwise could have been impractical. Appropriate methods for keeping groundwater resource sustainability in the study area have been suggested. [Journal of American Science. 2010;6(11):65-78]. (ISSN: 1545-1003).

Keywords: Groundwater vulnerability / DRASTIC / Tamtsag basin

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Structure Of Whey Protein Concequence For Dairy Industry  (Review)

Khorshid,M.A.and Fatma,A.M.

Dairy Department, National Research Center, Dokki , Cairo , Egypt

 khorshid88@hotmail.com

Abstract: Milk proteins play a range of roles which make dairy products and products containing dairy components are valuable. Theses include nutrition, physical functionality and breakdown under controlled condition to produce nutritional, functional or flavour full products. This article reviews the structure of whey protein concequence for dairy industry. [Journal of American Science. 2010;6(11):79-84]. (ISSN: 1545-1003).

Keywords: Milk proteins; dairy components; nutrition; review; dairy industry

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The Application Of Micro-Relief Meter For Soil Tillage Studies

M.A.Ghazavi1, A.Yosufvand­­1, B.Hosseinzadeh1

1 Department of mechanical farm machinery,University of Shahrekord, Shahrekord 115 Iran

Bahram_hs@yahoo.com

Abstract: Measuring the physical properties of soil provides a good opportunity for careful study of processes such as evaporation from the soil surface, formation of water runoff, sediments and erosion.  In this research, the change of some soil hydrological properties was studied in four kinds of primary soil cultivation activities by using a mechanical micro-relief meter. This study was conducted in a randomized complete block design. Data was collected in frames with 9025 cm2 area and 400 data height were collected for every frame. Measured soil properties were: Root Mean Square (RMS) of height data, superficial Profile Length Ratio (RZ) of soil roughness, Infiltration Recession Factor (RECS), plough depth, the volume of displaced soil, comparison of the area change in relation to superficial evaporation of soil. The RMS of height data was higher (p<0.05) with moldboard plow and modified disk plow than chisel and traditional ploughshares. The analysis of height data collected from plots showed that surface evaporation of soil moisture didn't differ by plowing with moldboard plow or traditional ploughshares, compared with two other ploughshares. This statistic was less than the recorded value of developed dish-like ploughshare (p<0.01). Also displaced soil mass by these two ploughshares was much more than chisel and traditional ones (p<0.01). The developed ploughshare prevents more evaporation. Therefore, plowing surface with this instrument provides high pot-hole store and penetration coefficient compared with other ploughshares. [Journal of American Science. 2010;6(11):85-89]. (ISSN: 1545-1003).

Keywords: RMS of height data, RZ, plough, height data, surface evaporation, pot-hole store

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Variations in some heavy metal concentrations in soil and Manihot esculanta tuber from the East and North eastern part of Nigeria     

S. T. Garba ,1*    J. T.,  Barminas, 2  and    A. H.  Santuraki,1

1Department of Chemistry, University of Maiduguri, Borno  state. P. M. B.  1069.  Nigeria.

2Department of Chemistry, Federal University of Technology Yola (FUTY), P. M. B. 2076. Adamawa state. Nigeria. stelagarba@yahoo.com

 Abstract: The levels of the heavy metals: Cr, Cd, Pb, Cu, Zn and Mn were determined in Manihot esculanta tubers and the soil used for its cultivation. This was done to asses the pollution level of the farmland and hence the safety status of Manihot esculanta tubers produced. Samples were collected from Konduga local government area of Borno state in the North East and from Umuahia local government area of Abia state in the East, all in Nigeria. These were treated and digested and the heavy metal concentrations were determined using Atomic Absorption Spectroscopy (AAS). The result obtained shows that, the samples from the North East had the highest levels of the elements Pb 30.14, Zn 88.65, Cd 3.15, Cu 16.00, Cr 6.74, and Mn 13.00 ppm in the soil sample while 5.47, 9.09, 5.05, 2.60, 3.37 ppm for the elements Pb, Zn, Cu, Cd, and Mn respectively were observed in manihot esculanta tubers sample. All these were found higher than what was observed in the soil and manihot esculanta samples collected from the East. Cr, was however, found below detection limits in manihot esculanta tuber samples from both the regions. [Journal of American Science. 2010;6(11):90-94]. (ISSN: 1545-1003).

Keywords: Health, Safety, Environment, Development, Borno State, Abia State, Nigeria

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Exploitation of palms wine in the municipality of Ze (Benin): socio-economic and physical impacts

Ade Romaric Herman1*, Bio Bigou Bani Leon2, Luo Zhaohui3

1.       School of Environmental Studies, China University of Geosciences, Hubei province, 388 lumo Road, 430074 Wuhan, P.R China, , 008613797056028

2.       University of Benin, (Abomey-Calavi),  General Secretary of university  Box: 526- Abomey- Calavi- Benin

3.       China University of Geosciences, Department of Environmental Sciences,  388 Lumo Road, Wuhan City, Hubei province, 430074, P.R. China

Abstract: The exploitation of palm wine is one of activities which many people from southern Benin particularly the municipality of Ze have engaged. This study aims to analyze the socio-economic and environmental impacts of the exploitation of palm wine in the municipality. The methodology consisted of collecting demographic, agricultural, socio economic data, processing and analysis of data collected in real area based on components of the Leopold matrix. The exploitation of palm wine has changed the agricultural landscape of the municipality of Ze.  The soil is becoming unproductive for food crops after a long fallow palm wine causing impoverished land due to overuse. The vegetation is endangered because of the rarity of certain plant species like Acacia sp (acacia), Antiaris africana, sapadi Blighia (bligia tasty), Chloroophora excelsa, Cola nitida, etc. But, in other aspect this activity is contributing to improve the living conditions of farmers and reduce the production of oil palm in this area. The production of alcohol provides employment to about 42% of the active population in the municipality of Ze, (municipality of Ze, 2007). At various levels of production that is from felling to alcohol distillation there are large numbers of people being employed. [Journal of American Science. 2010;6(11):95-102]. (ISSN: 1545-1003).

Key words: palm wine; sodabi; socio- economic and physical impact; municipality of Ze

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Sorption characteristics of copper in some calcareous soils of western Iran

A. R. Hosseinpur1 and  F. Dandanmozd2

1.Soil Sci. Dep. Shahrekord Univ. Shahrekord, Iran

2.Soil Sci. Dep. Bu-Ali Sina Univ. Hamadan, Iran.

E-mail: hosseinpur-a@agr.sku.ac.ir

Abstract:The environmental impact of metal additions to soil depends on its sorption ability. To evaluate the sorption of copper (Cu) on to some soils an experiment was conducted with ten  calcareous soils of Hamadan province in the west of Iran. Half g soil samples were equilibrated at 25±1 and 45±1°C with 25 ml of 0.01 M CaCl2 containing  0 to 30 mgL-1 Cu as CuSO4. Suspensions were centrifuged, filtered and concentration of Cu in the clear extract solution was calculated. The thermodynamic parameters viz. K, ∆G, ∆Hand ∆S were determined by using sorption data and concentration of Cu in equilibrium solution at two different temperature. Thermodynamic parameters revealed that Cu sorption increased as the values of K and ∆G increased with rise in temperature from 25 to 45 °C. The ΔGo values at 25 and 45°C were negative and ranged from -18.39 to -24.10 and -21.167 to -26.267 kJ mol-1 respectively. The values of enthalpy (ΔHo) and antropy (∆S)  were positive and ranged from 8.184 to 42.852 kJ mol-1 and 102.457 to 206.184 J mol-1 K-1 . The results showed that Cu sorption is a spontaneous process and endothermic reaction. The results also showed that calcareous soils can  sorb high amounts of Cu and that thermodynamic parameters are useful in describing Cu sorption. [Journal of American Science. 2010;6(11):103-108]. (ISSN: 1545-1003).

Keywords: Sorption isotherm; Calcareous soils; Thermodynamic parameter; Copper

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Association between Single nucleotide polymorphisms in Gallinacin genes and resistance to Marek's disease in White Leghorn chicken

yacoub, H. A*1, Galal, A2, El Fiky, S.A1 and Fathi, M. M 2,

1Cell Biology Department, National Research Center, Giza, Egypt

 2 Poultry Production Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

*Haitham_yakoub@yahoo.com

Abstract: Gallinacins are antimicrobial peptides that play a significant role in innate immunity in chicken. The aim of this study was to determine the relationship between candidate genes of innate immunity and resistance to Marek's disease and to predict whether the amino acids substitutions lead to produce new phenotypes. We used in current study two inbred lines of White Leghorn chickens, line 6 which selected for resistant to Marek's disease and line 7 which selected to susceptible to Marek's disease from ADOL, ARS,USDA. We examined Gal-1 and Gal-2 in current study by sequenced a 1.38 kb in two directions from two inbred lines (6 and 7). A total of 6 SNPs were identified within the sequenced regions. This equates to an SNP rate of 4.34 SNPs/kb, nearly to the previously reported 5 SNPs/kb across the entire chicken genome. The current study showed that the gallinacin genes are polymorphic because there are many single nucleotide polymorphisms (SNPs) in both inbred lines of White Leghorn chickens and some of these SNPs are nonsynonymous and others are synonymous and some of them are located in intronic region and the rest are in exonic region. All identified SNPs were intronic; except for Gal-1 was exonic resulting in amino acids changes which have a non-synonymous SNP resulting in amino acids alterations of asparagine to serine, histidine to tyrosine and tyrosine to serine, respectively. From SIFT (Sorting Intolerant from tolerant) program which used to predict whether an amino acids substitutions can affect protein function resulting in phenotypic effect , that is may be made the inbred line 7 of White Leghorn chickens are susceptible to Marek's disease rather than line 6. We are concluded that a new chromosomal region with effects on the response to Marek's disease in chickens was characterized in this study. Within this region, the SNPs in the gallinacin candidate genes could potentially be used in a marker assisted selection program to enhance the response to Marek's disease. Analysis of the gallinacin genes in the protective pathways of disease resistance has also opened the possibilities for therapeutic strategies using endogenous antimicrobial peptides. [Journal of American Science. 2010;6(11):109-114]. (ISSN: 1545-1003).

Keywords: single nucleotide polymorphisms, Gallinacin, genes, Marek's disease, resistance

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Endometrial Cytology and Bacteriological Isolates From Buffaloes With Retained Fetal Membranes and Their Effects on the Reproductive Efficiency

 

Amer H. A.1*, AbouZeid N. Z.2 and Barakat T. M.1

Department of Theriogenology1 and Infectious Diseases2, Faculty of Veterinary Medicine, Zagazig University, Egypt

samarmed84@yahoo.com

 

Abstract: This study aimed to determine if the buffaloes with retained fetal membranes (RFM) and without systemic involvement had an effect on the subsequent reproductive efficiency. One hundred buffaloes with or without placental retention were allocated into 4 groups, including  25 buffaloes  at day 15 post-calving had RFM (1st group),  25 buffaloes at 45 days post-calving had RFM (2nd group),  25 buffaloes without RFM  at day 15 post-calving as control (3rd  group ) and 25 buffaloes without RFM  at day 45 post-calving as control (4th group). The intrauterine perfusion fluid (10ml) was collected and examined bacteriologically and cytologically to evaluate the intrauterine environment. The reproductive parameters were determined in both buffaloes with or without retained fetal membranes. The detection rate of bacterial spp. was significantly (P<0.05) higher in buffaloes with RFM collected at day 15 after parturition than those in other groups. All 25 buffaloes with RFM at 15 days post-partum (100.0 %) showed positive results. From 22 of them (88.0%), more than one bacterial species was isolated. An Archanobacterium pyogenes (A. pyogenes) was isolated from 56.0% of buffaloes with RFM after 15 days post-calving. On the other hand, 5 (20.0%) out of 25 buffaloes with RFM at 45 days post-partum showed positive results. Nine out of 25 (36.0%) buffaloes without RFM at 15 days post-partum showed positive results. Moreover, 4 out of 25 (16.0%) control buffaloes at 45 days post-partum showed positive results. The bacterial species most frequently isolated was Lactobacillus spp. The number of buffaloes with ≥70% PMNs or ≤ 40% lymphocytes cells was higher (24/25, 96%) in the 1st group (RFM) at 15 days than those in 2nd group (RFM) at 45 days post-calving. The number of buffaloes with ≥70% PMNs or ≤ 40% lymphocytes cells was also significantly (P<0.01) higher in control group (17/25, 65%) at 15 days than those in control group (6/25, 24%) at 45 days. There were no significant variations among the groups of the buffaloes with retained placenta and the control groups at 15 and 45 days post-calving in postpartum uterine involution, the number of days from parturition to initial insemination, the number of days to conception and the number of services per conception. The overall conception rate was 15(60%) and 16(64%) in the RFM group, meanwhile, it was 19(76%) and 20(80%) in the control groups. It could be concluded that, in most buffaloes, the retained fetal membranes without systemic involvement had no major effect on the postpartum reproductive performance. [Journal of American Science. 2010;6(11):115-121]. (ISSN: 1545-1003).

 

Keywords: Buffaloes, Bacteriologically, Cytologically, Insemination, Conception.

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Toxoplasmosis in Naturally and Experimentally Infected Goats

AbouZeid N.Z.1, Amer H.A.2*, T.M. Barakat2 , Selim A.M.1 and El-Balkemey F.A.1

Department of Infectious Diseases1, Theriogenology2, Faculty of Veterinary Medicine, Zagazig University, Egypt

*samarmed84@yahoo.com

Abstract: One hundred slaughtered goats (2-3 years old) were used for diagnosis of toxoplasmosis in naturally infected goats, and 12 healthy pregnant and nonpregnant goats were used to study the pattern of toxoplasmosis as experimental study. Prevalence of toxoplasmosis in 100 slaughtered goats revealed that 29 (29%) and 27 (27%) were seropositive by LAT and IHA tests, respectively. There were agreement between LAT and IHA 97.3% in seronegative and 93.1% in seropositive sera in goats. There were complete concordance between LAT and bioassay in cats and mice. While the agreement between IHA result and bioassay in cat and mice was 93.1% in goats. Clinical examination of experimentally infected goats revealed that all goats had slight rise of body temperature; depression, anorexia, cough, muscular hyperthesia and diarrhea by day 5 and returned to normal by day 11. The age of fetus at the time of T. gondii infection is one of the known causes for the variability in clinical response. As infection of goats in early stage of pregnancy result in fetal reabsorption, while infection in mid pregnancy lead to abortion in one goat at 28 days post-infection and the other was aborted at 40 days post-infection. Moreover infections in late pregnancy resulted in delivery of viable kids. On the other hand controls goats were clinically normal and pregnant does were birth viable kids. LAT showed rapid response after 14 days post-infection, while IHA detected antibodies after 3 weeks post-infection. The antibody titers of both tests remained high until the end of experiment (48 weeks), while the titers were decreased around abortion or parturition and increased again after one week. Both LAT and IHA tests were insensitive in the pre-suckling kids from infected goats, whereas PCR gave positive results. In conclusion, PCR considered the most reliable tool for diagnosis of prenatal infection of toxoplasmosis, while LAT and IHA were considered unreliable tools for diagnosis of toxoplasmosis if they applied one week before or after kidding. [Journal of American Science. 2010;6(11):122-129]. (ISSN: 1545-1003).

Keywords: Prevalence, Toxoplasmosis, Goats, Abortion, Parturition.

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Photocatalytic Degradation of Monoazo and Diazo Dyes in Wastewater on Nanometer-Sized TiO2

S.A. Abo-Farha

Chemistry Department, Faculty of Science, Al-Azhar University (Girls), Naser City, Cairo, Egypt

samiaelhosieny@yahoo.com

Abstract: Advanced oxidation processes (AOPs) have proved very effective in treatment of the various hazardous organic pollutants in water. The photocatalytic degradation of two azo dyes, monoazo dye Acid Orange  10(AO10) and diazo dye Acid Red114(AR114) present in wastewater were studied. Homogeneous photocatalytic degradation of the two azo dyes with UV/H2O2 process was investigated. The rates of disappearance of the two azo dyes were monitored spectrophotometrically at the visible maximum absorption wavelengths. It was found that the rate of decolorization rises by increasing the initial dosage of H2O2 up to a “critical” value at which it is maximum and beyond which it is inhibited. The rates of reactions follow pseudo-first-order kinetics. Also heterogeneous photocatalytic degradation of the two azo dyes with UV/TiO2 (titanium dioxide) interface was investigated. The photocatalytic degradation rate depends on dye structure, dye concentration, TiO2 concentration and pH of the medium. The mechanism of the photodegradation process under UV-visible light illumination involves an electron excitation into the conduction band of the TiO2 semiconductor leading to the generation of very active oxygenated species that attack the dye molecules leading to photodegradation. Photocatalytic activity of TiO2 was examined by focusing on its enhancement by electron scavengers in the photocatalytic decomposition of the two azo dyes. The electron scavengers employed was inorganic oxidant such as H2O2, adequate dose of H2O2 led to a faster degradation of the two azo dyes in the TiO2 photocatalytic system. The fast decolorization of monoazo dye (AO10) than diazo dye (AR114) is an indication that, the number of azo and sulphonate groups in the dye molecule may be a determining factor for increasing the degradation rates. [Journal of American Science. 2010;6(11):130-142]. (ISSN: 1545-1003).

Keywords: Azo dyes; UV/H2O2 oxidation, Titanium dioxide; Photodegradation; Semiconductor.

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Phenotypic and genetic trends for milk production in Egyptian buffaloes

Fooda, T. A.; Kawthar A. Mourad and Gebreel, I. A

Animal Production Research Institute-Buffalo Breeding Research Department- Dooki- Giza - Egypt

Abstract: A total of 3495 records collected from 904 buffalo cows progeny of 174 sires and 470 dams through period from 1990 to 2008 in all Stations belonging to Animal Production Research Institute, Ministry of Agriculture were used to estimate the phenotypic and genetic trends for total milk yield. LSM for total milk at different year of calving ranged between 1334 kg and 1692 kg, 1028 kg and 1561 kg, 1209 kg and 1633 kg, 1355 kg and 1415 kg and 1137 kg and 1355 kg for El-Nattafe El-Gidid (NG), El-Nattafe El-Kadim (NK), Mahalet Mousa (MM), Gemiza (G) and Sids (S) stations, respectively. Estimates of the positive breeding value (BV, %) at different year of calving ranged between 40 % and 52 %, 31 % and 52 %, 40 % and 56 %, 37 % and 55 % and 45 % and 59 % for NG, NK, MM, G and S stations, respectively. Annual phenotypic trend for milk production ranged between -11.7 kg and +36.7 kg for S and NK stations, respectively. While, the annual genetic trend ranged between -0.16 kg and +0.6 kg for G and NG stations, respectively. The results of the present study showed that there are increased of improvement of phenotypic and genetic trend in all MM farms from 1990 until now. [Journal of American Science. 2010;6(11):143-147]. (ISSN: 1545-1003).

Keywords: buffalo, phenotypic trend, genetic trend, breeding value and milk production

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Computer Aided Design, Synthesis and Biological Evaluation of Novel Acridine Derivatives a Topoisomerase I Inhibitors

Gamil Mahmoud El Taliawi1, Enayat Ibrahim Ali1, Gehan Hegazy Hegazy*1, Nasser S. M. Ismail2 and Walaa Ramadan1

1Department of Pharmaceutical Chemistry, Faculty of Pharmacy Cairo University, 2 Pharmaceutical Chemistry Department, Faculty of Pharmacy Ain Shams University, Egypt.

*gehan_hegazy@yahoo.com

Abstract: A series of novel 9- anilinoacridines was designed and their molecular docking studies into the active site were examined as topoisomerase I inhibitor. Several compounds showed significant high simulation docking score. The designed compounds were synthesized and biologically evaluated against mammary carcinoma cell line (MCF-7), where compounds 8,11e,11f,13b,14b,14e and 14f showed significant inhibitory activity at a concentration 10μg/mL). It appears that the in vitro activity of compounds 8,11e,11f,13b,14b,14e and 14f were consistent with their molecular modeling results, and compound 14b showed the highest activity with IC50 value of 7.8 µg. [Journal of American Science. 2010;6(11):148-158]. (ISSN: 1545-1003).

Keywords: Molecular docking, Acridine derivatives, Antitumor

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Effect of Probiotic (Saccharomyces cerevisiae) Adding to Diets on Intestinal Microflora and Performance of Hy-Line Layers Hens

Saadia M. Hassanein1 and Nagla K. Soliman2

1Microbiology Dept, Faculty of Science, 2Poultry Production Dept., Faculty of Agriculture, Ain Shams University, Cairo, Egypt.

Abstract: As experiment was conducted to evaluate the affect of adding various levels of a live yeast to laying hen diets on their laying and feeding performance, egg shell, egg components and some blood constituents, as well as the intestinal microflora make-up. This were studied to validate the mode of a live yeast action in improving laying hens performance. For this purpose 75 Hy line (W-36) white layers were sited from 70 to 79 week of age in individual cages and randomly distributed into five experimental groups of 15 layers each. The individual hen was represented as an experimental unit. The five experimental groups were fed on five graded levels of a live yeast as 0.0% (control), 0.4%, 0.8%, 1.2% and 1.6%. The main results indicated an increase in egg production percentage of layers fed with 0.4% and 0.8% a live yeast which recorded 83.4% and 80.6% respectively compared with 74% of control which was similar to the groups of layers fed 1.2% (74.9%) and 1.6% (74.6%). Average egg weight was not influenced by adding yeast into diets. Egg mass results were parallel to these of egg production where the values of 46.7, 51.0, 50.2, 48.3 and 46.1 g egg/hen/day were recorded for the group of birds fed with 0%, 0.4%, 0.8%, 1.2% and 1.6% a live yeast respectively. Egg albumen and egg yolk were affected significantly. There was a slight improvement in egg shell thickness and percentage. Feed intake values were approximately similar within the different treatments. Feed conversion ratios (g feed/g egg) of layers fed yeast levels of 0.4% (2.08) and 0.8% (2.07) were better than the control group (2.27). Blood total protein levels of birds fed 0.4% (3.82), 0.8% (3.65) and 1.2% (3.97) yeast were lower than the control (4.16), while the value of 1.6% yeast (4.16) was slightly higher than control. Blood albumen levels were parallel to those of blood protein while blood globulin values were not affected. Blood cholesterol levels of layers fed yeast-supplemented diets were lower than the control. Blood total lipids were not affected by treatments. Ileal content pH of layers fed 0.8% and 1.2% yeast levels was lower than the control. Microbiological examination of ileal content indicated an obvious reduction in bacterial total count. While Lactobacilli bacterial count was increased. There were reductions in bacterial strains of Escherichia coli (E.coli), Klebsiella sp., Staphylococcus sp., Micrococcus sp., Campylobacter sp., and Closterdium perfringers of layers fed various yeast levels. The results of this study suggest adding live yeast at 0.4% or 0.8% into laying hen diets can enhance the productive performance and nutrients utilization via the inhibitory effect of yeast against pathogenic bacteria. [Journal of American Science. 2010;6(11):159-169]. (ISSN: 1545-1003).

Keywords: yeast level, laying hen, egg production, ileal microflora, blood constituents.

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Properties of enterotoxigenic S. aureus Isolated from mastitic cattle and buffaloes in Egypt

Jakeen Kamal Abdel Haleem El-Jakee1, Emad Rizkalla Zaki2, Randa Samy Farag2

1-Microbiology Department Faculty of Vet. Medicine Cairo University

2-Buffaloes Diseases Department, Animal Health Research Institute, Doki, Giza.

jeljakee@yahoo.com

Abstract: Enterotoxigenic S. aureus in milk posses a potential health hazard to consumers. In this paper 106 S. aureus isolated from cow and buffalo milk samples were investigated for production of enterotoxins. RPLA results showed high incidence of type C enterotoxin followed by type A and type B with incidence of 34 (32.1%), 19 (17.9%) and 15 (14.2%) respectively. Toxigenic S. aureus isolates produced golden yellow, creamy and white colonies on agar in percent of 69.11%, 27.94% and 2.94% respectively. Regarding to hemolytic activity on sheep blood agar, 92.65% of toxigenic S. aureus isolated from bovine milk samples were hemolytic. A correlation exists between toxigenic isolates and coagulase and DNase production. On crystal violet agar medium. 23.53% of the S. aureus isolates yielded yellow colonies, 64.71% yielded violet colonies, while 11.76% yielded white colonies from the toxigenic S. aureus isolates. It is clear that most of bovine isolates yielded violet colonies on the medium. Out of 68 isolates of toxigenic S. aureus isolates 51 (75%) showed SpA by agglutination test positive. Results obtained showed 100% agreement between RPLA and PCR techniques. [Journal of American Science. 2010;6(11):170-178]. (ISSN: 1545-1003).

Keywords: S. aureus, mastitis, enterotoxins, RPLA, PC

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Hydro-Thermal Safety Control of Karun-1 Dam under Unusual Reservoir Level Reduction 

Mojtaba Labibzadeh 1

1. Department of Civil Engineering, Faculty of Engineering, Shahid Chamran University, Ahvaz, Iran

Labibzadeh_m@scu.ac.ir

Abstract: Karun-1 dam safety was examined through carrying over a 3D finite elements analysis. The dam as well as its foundation and abutments have been modeled in a relatively exact manner. Furthermore, the vertical contraction joints were simulated in calculations. Hydrostatic, gravity and thermal effects have been taken into account as the load collections. 10m reduction of reservoir level from normal water level of the dam reservoir was applied in the modeling and the possibility of initiate and development of cracks in dam body was investigated by means of monitoring of principal stresses. The obtained results showed that mentioned possibility existed and the downstream face of the dam in vicinity of the abutments near to crest level probably experiences the tensile cracks. [Journal of American Science. 2010;6(11):179-184]. (ISSN: 1545-1003).

Keywords: Arch dam, Thermal, Contraction joints, Cracks, Dam safety

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Prevalence of HBV Genotypes in Egypt among Hepatitis Patients

Iman A.El Aziz Khaled *1, Ola M. Mahmoud1, Abeya F.Saleh1, and  Emad A. Baioumi2

1Haematology &Blood Bank, 2Tropical Medicine,   Theodor Bilharz Research Institute (TBRI).Cairo, Egypt; *iman_khaled@yahoo.com

Abstract: Phylogenetic analysis has led to the classification of hepatitis B virus into eight genotypes, designated A to H. The genotypes have differences in biological properties and show heterogeneity in their global distribution. These attributes of the genotypes may account not only for differences in the prevalence of hepatitis B virus mutants in various geographic regions, but also makes them responsible for differences in the clinical outcome and response to antiviral treatment in different population groups. Africa is one of the highly endemic regions of HBV with five genotypes (A-E) identified. Almost all patients in the Mediterranean area are infected with genotype D. However, there is little information of genotype distribution in Egypt. A total of 140 Egyptian patients with hepatitis B surface antigen (HBsAg) positive were enrolled in this study. Of the 140 patients, only100 patients were HBV DNA positive and only these were included in the study. They were classified in to 20 patients with acute hepatitis (AH), 75 patients with chronic active hepatitis (CAH) and 5 patients with hepatocellular carcinoma (HCC)]. HBV genotypes were determined using INNO-LiPA methodology which is based on the reversed hybridization principle. Results: This study showed that genotype D constituted 87% of the total infections (75% CAH, 7% AH & 5% HCC). The other 13% showed mixed infections of D/F. Conclusion: These findings show that the most prevalent genotype in Egypt is genotype D especially in CAH and HCC patients while the mixed type D/F is mostly encountered in AH. [Journal of American Science. 2010;6(11):185-190]. (ISSN: 1545-1003).

 Keywords: Phylogenetic, Genotypes , Hepatocellular

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Cytogenetic Study of the Effect of Schistosoma mansoni Infection on Human Peripheral Blood Lymphocytes and the Role of β-Carotene and Vitamin E in Modulating this Effect.     

Mervat S.El-Ansary1, Imam A.Khaled2, Abeya F. Saleh2, Ola M.Mahmoud2, Emad A. Baioumi3, Heba A.Bakr4

1Immunology (Cairo University), 2Haematology (TBRI), 3Hepatology (TBRI), 4Science (Ain Shams University), Cairo Egypt, iman_khaled@yahoo.com

Abstract: Aim: This study has been made to determine the potential genotoxicity of Schistosoma mansoni on lymphocytes of infected patients using different mutagenic end points. The protective role of antioxidants pro vitamin β-carotene and vitamin E in minimizing these genotoxic effect was also studied. The study focused on the effect of schistosomiasis on the induction of sister chromatid exchange (SCEs) and other chromosomal aberrations. Patients and Methods: This work was conducted on 24 Schistosoma mansoni infected patients and 10 healthy adults as a control group. Lymphocytes from peripheral blood of patients and control group were used for culture and subsequent cytogenetic studies. Results: The results indicated that schistosomiasis was genotoxic in all examined tests. It induced a significant increase in the percentage of structural chromosomal aberrations and the frequency of SCEs. It also inhibited cell division and caused cell cycle delay. Lymphocyte cultures of S. mansoni patients treated with 10 mg/ml β-carotene or 20 mg/ml vitamin E showed a significant decrease in the percentage of structural chromosomal aberrations and the frequency of SCEs. Conclusion: schistosomiasis has a genotoxic effect on peripheral blood lymphocytes. The use of the antioxidants β-carotene and vitamin E can be considered a promising approach not only toward inhibiting the genetic damage of schistosomiasis but also as prophylactic agents against infection with S mansoni. Furthermore, higher doses of antioxidant drugs, β-carotene and vitamin E, should be tried as an adjuvants to conventional therapy in a trial to improve treatment of schistosomiasis. [Journal of American Science. 2010;6(11):191-202]. (ISSN: 1545-1003).

Key words: Schistosomiasis, β-carotene, vitamin E, chromosomal aberration

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BIOCHEMICAL PATTERN FOR HEPATITIS C AND ACUTE LYMPHOBLASTIC LEUKEMIA IN HUMAN SERA 

Abulyazid1, I., Mohga S. Abdallah2, Hayate I. Sharada3 and Sama H. Okasha4 

1, Molecular Biology Department, Atomic Energy Authority

2, 3, 4 Chemistry Department, Faculty of Science, Helwan university 

Abstract: Present experimental work aimed to show role of the molecular biology in diagnosis of hepatitis C liver disease (HCV) and acute lymphoblastic leukemia (ALL) which occur as a result of the disturbances of protein and enzymes fractions at the molecular level. The study carried out using vertical slab gel electrophoresis for detection of the protein pattern, catalase and peroxidase. Protein fractionation of the control samples produced 13 bands with Rf ranged between 0.17 and 0.96 and (amount, 3.14 - 7.24). Comparing hepatitis C with control one out of these 13 bands are completely disappeared at Rf 0.86 (amount 9.34). Ten bands appeared to be common bands in all HCV samples except one sample only nine common bands were produced while the band number ten was disappeared. The data showed that 5 characteristic bands were produced. One from these five bands determined at Rf 0.7 in all HCV sera samples except the first sample. Comparing leukemia samples with control only two were considered as common bands. These bands completely appeared in all sera samples. On the other side one band was completely disappeared in all leukemia samples. The rest bands distributed between different leukemia samples. 15 bands produced as characteristic bands. Electrophoresis pattern for catalase mentioned that six bands were produced in control samples. When hepatitis C compared with the control showed that two out these six bands were completely disappeared and other all HCV four bands considered as common bands. The amount of catalase enzyme completely decreased in all bands. In leukemia five common bands were produced with the appearance of one characteristic band, from the other side one band was disappeared. A documentation of peroxidase pattern data showed that tow common bands were appeared with Rf 0.1 and 0.33, the amount of these two bands were decreased when the amount of HCV compared with control in the same rows.  In leukemia there is only one common band was produced with appearance of a three characteristic bands.  [Journal of American Science. 2010;6(11):203-216]. (ISSN: 1545-1003). 

Keywords: HCV, Acute lymphoblastic leukemia, Protein electrophoresis, catalase, peroxidase

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Serum resistin levels and haemostatic changes in experimentally induced diabetic and high fat fed rats 

Mohammad I. Hoseen, Mai M .Hassan, Dalia I. Abd-Alaleem and Eman M. Faragallah. 

Department of physiology, Faculty of medicine, Zagazig University. 

Abstract: Adipose tissue is considered as an active endocrine gland that affects many aspects of body homeostasis. Adipose tissue derived molecules ‘‘adipokines” regulate energy homeostasis, dietary behavior, as well as insulin sensitivity and immunity; it refers to leptin, adiponectin, resistin, apelin, visfatin and omentin. Resistin is a cysteine-rich adipokine that is released by adipocytes and macrophages and has been involved in the development of insulin resistance in rodents. Moreover a strong link between diabetes, hypercoagulability and thrombogenesis, had been recognized for decades. Aim: In a trial to identify any possible relationship between resistin levels and some haemostatic changes in streptozotocin-induced diabetic and high fat diet-fed rats (HFD); the present work had been carried out. Design: A total number of 40 adult male albino rats were divided into 2 main groups: Group I (n= 24): To study the effect of streptozotocin-induced type 1 diabetes and was further divided into 3 equal subgroups (n= 8 in each) and survived for 30 days: Ia: (control group), Ib: (experimental diabetic non-treated group (by a single i.p. injection of streptozotocin (65mg/Kg B.W), Ic (experimental diabetic group treated with insulin). Group II (n= 16) : To study the effect of high fat diet and was further divided into 2 equal subgroups (n= 8 in each) and survived for 7 weeks: IIa: (control group),IIb (high fat diet fed  (58% fat). In all groups, serum levels of glucose, insulin, resistin, total cholesterol(TC), triglycerides (TG), HDL, LDL, BT, WBCT, PT, aPTT, plasma fibrinogen level, plasma D-dimmers level and platelet count were measured. Results:  The results of this study showed a significant decrease in serum resistin levels (p<0.001) in streptozotocin-induced diabetic group in comparison with its control group and insulin-treated group. Moreover, no significant correlation could be detected between resistin levels and any of measured parameters in these groups except the significant positive correlation with body weight at the end of experimental period. In addition, our study revealed a significant increase in serum resistin levels (p<0.001) in HFD-fed group in comparison with its controls, which was correlated positively and significantly  with body weight, serum glucose levels, insulin levels and HOMA-IR index (p<0.001), atherogenic lipid profile and markers of hyper-coagulability (except for platelet count). Conclusion: No role for resistin in metabolic and haemostatic changes in type 1 diabetic rats was detected. Although, hyperresistinemia may represent a link between metabolic signals, atherogenesis, and hypercoagulability in type 2 diabetic rats. However, further studies are needed to clarify this relationship in human cardiovascular diseases. [Journal of American Science. 2010;6(11):217-227]. (ISSN: 1545-1003). 

Keywords: Resistin, Streptozotocin, high fat, diabetes, heamostasis

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Analytic Investigation and Numeric Prediction for Biodynamic Response of the Seated Human Body

Mostafa A. M. Abdeena, W. Abbasb

a Dept. of Eng. Mathematics and Physics, Faculty of Eng. Cairo University, Egypt

bEng. Physics and Mathematics Dept., Faculty of Eng. (Mataria), Helwan University, Egypt

msotafa_a_m_abdeen@hotmail.com; wael_abass@hotmail.com

Abstract: The biodynamic response behaviors of seated human body subject to whole-body vibration have been widely investigated. The biodynamic response characteristics of seated human subjects have been extensively reported in terms of apparent mass and driving-point mechanical impedance while seat-to-head vibration transmissibility has been widely used to characterize response behavior of the seated subjects exposed to vibration. These functions (apparent mass, driving-point mechanical impedance) describe to-the-body” force–motion relationship at the human–seat interface, while the transmissibility function describes “through-the-body” vibration transmission properties. The current study proposed a  4-DOF analytic biomechanical model of the human body in a sitting posture without backrest in vertical vibration direction to investigate the biodynamic responses  of different masses and stiffness. Following the analytical approach, numerical technique developed in the present paper to facilitate and rapid the analysis. The numerical analysis used here applies one of the artificial intelligence technique to simulate and predict the response behaviors of  seated human body for different masses and stiffness without the need to go through the analytic solution every time. The Artificial Neural Network (ANN) technique is introduced in the current study to predict the response behaviors for different masses and stiffness rather than those used in the analytic solution. The results of the numerical study showed that the ANN method with less effort was very efficiently capable of simulating and predicting the response behaviors of seated human body subject to whole-body vibration. [Journal of American Science. 2010;6(11):228-239]. (ISSN: 1545-1003).

Keywords: Biodynamic response, Analytic seated human body model, Numerical simulation model, Artificial Neural Network.

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Design Synthesis of New Peptide Derivatives and Evaluated DNA Binding Activity, Anticancer and Antimicrobial Activity.

A. A.  EL-HENAWY

Chemistry Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo-Egypt.

Abstract: Recently, sulfonamides have been reported to show significant antitumor activity in vitro and/or in vivo. There are a variety of mechanisms for the anticancer activity. The present work reports the synthesis some novel peptide sulfadiazine derivatives, this may play a role in their anticancer activity. All the newly synthesized compounds were evaluated for DNA binding activity and antimicrobial activity, some synthesized compounds showed high DNA binding activity and antimicrobial activity.  Some selected  compounds  were  evaluated  for  anticancer  activity  against  breast cancer  cell line  (MCF7)  in  vitro. All selected compounds showed interesting cytotoxic activities compared to a reference drug. [Journal of American Science. 2010;6(11):240-249]. (ISSN: 1545-1003).

Keywords: Peptide; Anticancer; Antimicrobial; DNA Binding

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Amelioration Productivity of Sandy Soil by using Amino Acids, Sulphur and Micronutrients for Sesame Production

Salwa, A.I. Eisa; Mohsen. M. Abass and S.S. Behary
Soils, Water and Environment Research Institute, ARC, Egypt
 

Abstract: A field experiment was carried out at Ismailia Agricultural Research Station, Ismailia Governorate, Egypt or two successive summer seasons 2008 and 2009 using sesame (Giza 32) to study the effect of elemental sulphur as soil application, amino acids and micronutrients (Fe, Zn, Mn) as foliar spray and their interactions by concentration (A0) zero and (A1) 2 g/l, for amino acids, micronutrients Fe, Zn and Mn were added as mixture at rates of zero (T0) & 450, 225, 225 µg g-1 (T1) and 900, 450, 450 µg g-1 (T2). While elemental sulphur was added at the ates of zero (S0) & 0.5 Mg S/fed (S1) and 1.0 Mg S/fed (S2), on sesame yield, its components and seed quality. Data indicated that, plant height (cm) significantly affected by all applied treatments. The tallest plant height (213.3 cm) achieved upon treating by A1 + 1.0 Mg S/fed + 900, 450, 450 µg g-1 Fe, Zn, Mn by rate of increases 48.0% over control. Data also show that there were significantly increases in the whole plant weight with increasing application of sulphur as soil application and micronutrients as foliar spray. The highest plant weight were achieved upon reating by A1 + 1.0 Mg S/fed + 900, 450, 450 µg g-1 (Fe, Zn, Mn) by rate of increases 50.04% over control. A significantly increase in the seed sesame yields, the highest seed yields were achieved upon treating by A0 + 1.0 Mg S/fed + 900, 450, 450 µg g-1 (Fe, Zn, Mn) by rate of increases 89.80% over control. For seed quality data show that an increases in P and K%, Fe, Zn and Mn µg g-1 ,oil and protein%, oil and protein yield upon treating by A1 + 1.0 Mg S/fed + 900, 450, 450 µg g-1 (Fe, Zn, Mn). Generally, a combined application of amino acid with micronutrient Fe, Zn, Mn in the presence of elemental sulphur significantly increased the sesame yield; improved nutrition and ncreased seed quality; except the seeds yield whither the highest amount for seeds yield occurred in absence of amino acids; it was probably related to the physiological actions of amino acids and micronutrients. [Journal of American Science. 2010;6(11):250-257]. (ISSN: 1545-1003).


Key words: Amino acids- sulphur-micronutrients-sesame-sandy soil.

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Comparative Study of Software Engineering Processes in Egyptian Cmmi Companies

 

Alaa El-Din Hamouda and Mohammad Abdrabo Elwahsh

Computers & Systems Engineering Dept.,Al-Azhar University Cairo, Egypt.

Alaa_ham@gega.neteng.md.elwahsh@gmail.com

 

Abstract: The Egyptian government has paid special attention to the software industry as Egypt to provide it with a competitive advantage that makes this emerging industry promising. Thus, the State has supported the Egyptian companies to make use of the Capability Maturity Model Integration (CMMI). Since 2009, more than thirty companies obtained the CMMI at different levels. However, these companies suffer from lack of a mechanism to exchange experience and information among themselves although they could be similar in the culture of their engineers and perhaps in the nature and size of their software projects. So, we provide in this research a survey to gauge the quality of methods, tools and processes used in these Egyptian companies winning the CMMI. Then we analyzed the results to reach the recommendations aimed at enriching the software industry in Egypt. [Journal of American Science. 2010;6(11):258-263]. (ISSN: 1545-1003). 31

 

Keywords: CMMI in Egypt, software engineering processes, survey.

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Margin Assessment and Fracture Resistance of Adhesively Luted Ceramic Crowns

Jylan F. ElGuindy1, Dina H. Mostafa*2 and Mona A. El Agroudi1

1Fixed Prosthodontics Department and 2Biomaterials Department, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt.

Abstract: Objectives: The aim of this study was to investigate the effect of different adhesive systems on the vertical marginal gap distance and the fracture resistance of lithium disilicate based crowns.

Methods: Forty premolars were prepared to receive forty e-max crowns. The crowns were divided into 4 groups (N=10 each) according to the adhesive luting systems. Group (U): using RelyX Unicem resin cement (self-adhesive system). Group (V): Variolink II (total-etch system). Group (GU) and group (GV): application of G-bond (self-etch) on dentin preceded previously used adhesive systems. A stereomicroscope was used to record the vertical marginal gap distance before and after cementation. The crowns were subjected to cyclic loading and fracture resistance test. Data were statistically analyzed using One-way Analysis of Variance (ANOVA) SPSS 15.0. A scanning electron microscope was used to qualitatively examine the dentin/resin interface. Results: Groups (GU) (67.6 ± 5.8 µm) and (GV) (68 ± 6.4 µm) recorded the significantly lowest vertical marginal gap, followed by group (V) (82 ± 6.8 µm). Group (U) showed the highest marginal inaccuracy (114 ± 6.4 µm). Group (GU) recorded the significantly highest fracture resistance (2840.5 ± 3.8 N), followed by group (GV) (2411.3 ± 3.3 N) and group (V) (2365.8 ± 3.6 N). Group (U) showed the lowest results (2270.9 ± 3.4 N). Conclusions:  Ceramic restorations luted with total-etch system offer better vertical marginal gap distance and fracture resistance than restorations luted with self-adhesive system.  Treatment of the dentin surface prior to the application of the bonding system is efficient. [Journal of American Science. 2010;6(11):264-273]. (ISSN: 1545-1003).

 Keywords: Adhesives, marginal gap, fracture resistance, all-ceramics

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Do Desensitizers Affect the Retention of Questionable Preparations?

Jylan F. ElGuindy1, Dina H. Mostafa*2 and Mona A. El Agroudi1

1Fixed Prosthodontics Department and 2Biomaterials Department, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt.

Abstract: Objectives: This study aimed to investigate the effect of different desensitizers on the retention of short and over-converged preparations. Methods: Eighty molars were prepared with 3mm occluso-cervical height and 24 degrees convergence angle. Nickel-chromium copings were cast with a loop at the occlusal surface for tensile loading after cementation. The copings were assigned to two groups (N=40 each) according to the cement used. Group 1: resin cement (Duolink). Group 2: glass-ionomer (Ketac-Cem). Each group was assigned to four subgroups (N=10 each) according to desensitizers used prior each cement. Subgroup I: control (untreated), subgroup II: Gluma Comfort + Desensitizer, subgroup III: Oxalate (Bisblock) and subgroup IV: Fluoride varnish (Flor-Opal). The retention was determined by uniaxial tensile mode of force. Two-way Analysis of Variance (ANOVA) SPSS 16.0 was used to assess cements, desensitizers and their interactions on copings retention. Results: Resin group: Oxalate (212.10±7.41N) showed the significant highest mean of retention, followed by Gluma (201.52±6.93N), then control (177.52±6.14N). Fluoride (153.80±6.03N) recorded the lowest mean. Glass-ionomer group: control (135.54±4.58N) and Oxalate (132.62±4.84N) recorded the significant highest mean, followed by Gluma (126.84±4.75N). Fluoride (101.96±6.34N) recorded the lowest mean values. Conclusions: With questionable preparations, fluoride desensitizer drastically affected the retention of both cements. Oxalate and Gluma enhanced the retention with resin cement. Oxalate desensitizer can be efficiently used with glass-ionomer. [Journal of American Science. 2010;6(11):274-283]. (ISSN: 1545-1003).

Keywords: retention; Nickel-chromium coping;  occlusal surface; Fluoride varnish

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Accuracy of working casts and dies produced by fast-setting polyvinyl siloxane impressions

Mona El-Agroudi MD DDS1 and Eman Essam, MD DDS2

  Assistant professor of Fixed Prosthodontics, Faculty of Oral and Dental Medicine, Cairo University. , Egypt.

2 Lecturer of Fixed Prosthodontics,  Faculty of Oral and Dental Medicine, Al Azhar University. , Egypt.

Abstract: Purpose: This study aimed to evaluate the effect of spacer on the accuracy of working casts and dies produced from fast-setting polyvinyl siloxane impressions.

Materials and Methods: Twenty Impressions of the mandibular arch of a modified Dentoform master model incorporating a stainless steel circular crown preparation were made, using a fast-set Polyvinyl siloxane (Affinis perfect impressions Coltene/Whaladent AG) using 2/step impression technique with and without a spacer. Gypsum working casts and dies were produced from the poured impressions. Measurements of the master model and working casts were carried out including anteroposterior (AP) and cross-arch (CA) dimensions. The stainless steel circular crown preparation incorporated within the master model was also measured in buccolingual (BL), mesiodistal (MD), and occlusogingival (OG) dimensions and compared to measurements from recovered gypsum dies. Linear measurements were made using a measuring stereomicroscope. Results: Double impression technique without spacer showed statistically significant higher mean percent relative change than double impression technique with spacer. With each technique, the means percent relative change in die measurements showed statistically significant higher mean values than cast measurements. There was no statistically significant difference between means percent relative change in the BL and MD dimensions which showed the statistically significant highest mean values. The means percent relative change in the OG dimension showed the statistically significant lowest mean value. Conclusion: Accuracy of fast-setting polyvinyl siloxane impression material was favorably affected with the use of spacer, as the space resulted from contraction of the putty material was not enough to produce accurate detail reproduction by the light material. The working dies; from the fast- setting polyvinyl siloxane impression material without spacer demonstrated an increase in (mesio-distal and bucco-lingual) dimensions, while for cast dimensions, there was no difference between the two techniques. [Journal of American Science. 2010;6(11):284-292]. (ISSN: 1545-1003).

Keywords: dies; fast-setting; polyvinyl siloxane

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Kinetics and Thermodynamics of Oil Extraction from Jatropha Curcas in Aqueous Acidic Hexane Solutions

Sh. K. Amin, S. Hawash, G. El Diwani*, and S. El Rafei

Chemical Engineering and Pilot Plant Department, National Research Center, Cairo, Egypt.

*geldiwani@yahoo.com

Abstract: Jatropha oil curcas (JOC) extraction was performed in aqueous HCl, H2SO4, and H3PO4 solutions with n–hexane (C6H14) at 30, 40, 50, and 60 °C using 10 gm of Jatropha seeds over 1 hours with 10 minutes sampling intervals. The optimum acid concentration was 15 % by weight for each acid, and the highest oil yield was obtained in the extraction procedure with n–hexane containing HCl. The extraction process was observed with regard to the percent oil yield versus time, and the reaction order was found to be first–order kinetics by the differential method. The activation energy for the oil extraction kinetics of Jatropha seeds with 15 % HCl was Ea = 26.6763 kJ/mol, and the activation thermodynamic parameters at 60 °C were ΔH = 23.908 kJ/mol, ΔS = - 239.927 J/mol.K, and ΔG = 103.803 kJ/mol. The enthalpy value was ΔH = 0.1586 kJ/mol, and the other thermodynamic parameters at 60 °C were calculated to be ΔS = 15.275 J/mol.K, and ΔG = - 4.928 kJ/mol. [Journal of American Science. 2010;6(11):293-300]. (ISSN: 1545-1003).

Key words:  kinetics, thermodynamics, oil extraction, Jatropha curcas

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Methodology for Selective Adsorption of Lithium Ions onto Polymeric Aluminium (III) Hydroxide

S. Hawash, E. Abd El Kader and G. El Diwani*

Chemical Engineering and Pilot Plant Department, National Research Center, Cairo, Egypt. *geldiwani@yahoo.com

Abstract: The recovery of lithium as lithium aluminate from Egyptian bitterns was investigated. Studies were performed on synthetic Li+ solution and on three high – salinity end brines which contain Li+ of concentrations varying between 5.5- 19.5 ppm. Pretreatment with a mixture of Na2SO4- Na2CO3 is achieved to precipitate BaSO4, SrCO3, CaCO3 and possibly MgCO3. A co-precipitation method was employed using aluminum salt as (AlCl3.6H2O). Lithium ion is adsorbed onto aluminum hydroxide, which is freshly produced by adding AlCl3.6H2O and Na OH to the brines at Al3+ / Li+ molar ratio≈ 5-7. Results obtained indicate that high Li+ adsorption was performed at pH = 6-7 for Alexandria-Arish and Emissal salines, even for small concentration of aluminum salt added. Also, Lithium ions uptake decreased with increasing adsorption temperature from 10ºC to 30ºC but over 30ºC increase in temperature does not affect lithium uptake on Al(OH)3, which proved that the process is physical adsorption. Equilibrium isotherms have been determined for the adsorption of Li+ onto Al (OH)3 at 30ºC and pH= values (5 to 9), the maximum adsorption capacity of Al(OH)3 at 30ºC and pH = 9 is 123 mg/gm. The results indicated that applied isotherms were shown to be "favorable" and were fitted with Langmuir and Freundlich isotherms. Li+ desorption from Al(OH)3 was investigated using hydrofluoric acid (HF) or sulphuric acid (H2SO4) with different concentrations, and results obtained showed that HF is more efficient than H2SO4 concerning Li+ desorption. From the obtained results, Li ion can be recovered successfully from bittern and saline solutions. [Journal of American Science. 2010;6(11):301-309]. (ISSN: 1545-1003).

Keywords: lithium; lithium aluminate; hydrofluoric acid (HF); sulphuric acid (H2SO4; saline solution

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The Effects of Dietary Egyptian Propolis and Bee Pollen Supplementation against Toxicity if Sodium Fluoride in Rats

Fatma A. Khalil and Nora M. El-Sheikh

Biochemistry and Nutrition Department, Women's College, Ain Shams University, Cairo, Egypt.

Abstract: Propolis and bee pollen are substances produced by honey bees its components are strong antioxidant and free radical scavengers. The present study aimed to study the protective effects of propolis and bee pollen supplementation against toxicity of sodium fluoride in rats. After the end of experimental period, the rats sacrified and biochemical analysis were carried out. The results showed that the administration of fluoride (F) alone causes significant increase of malondialdehyde (MDA) level and significant decrease of antioxidant system as erythrocyte superoxide dismutase (SOD) activity and reduced glutathione (GSH) levels in blood and brain. Also F causes significant increase alkaline phosphatase (ALP) activity, urea, creatinine, sodium and potassium levels. And significant decrease total protein, calcium, magnesium and phosphorus levels as compared to control group (P < 0.05). Whereas administration of propolis or bee pollen with F led to significant decrease in MDA level and significant increase in SOD activity, GSH levels in blood and brain. And significant decrease ALP activity, urea, creatinine, sodium and potassium levels in serum. The propolis or bee pollen enhanced total protein, calcium, magnesium and phosphorus levels in serum as compared to F group alone.

In conclusion; supplementation of natural antioxidant (propolis or bee pollen) during Fluoride administration, facilitate reduction of the toxic effects and enhanced the antioxidant system, the levels of minerals is serum. [Journal of American Science. 2010;6(11):310-316]. (ISSN: 1545-1003).

Keywords:  Propolis, bee pollen, sodium fluoride, rats, antioxidant system minerals.

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Diet Selection, Feed Intake Capacity and Performance of Growing Female Camels:  Effects of Type of Roughage and Level of Concentrates Offered

M.F.A. Farid, A.M. Abdel-Wahed, Safinaz M. Shawket* and N.I. Hassan

Animal Nutrition Department, Desert Research Centre, Al-Matareya, Cairo, Egypt.

drsafinazshawket@hotmail.com*

Abstract: The feeding trials were intended to investigate diet selection, feed intake capacity (FIC) and animal performance when concentrates (corn grains and commercial concentrates mixture) and roughages (Atriplex, clover hay or rice straw) were fed ad lib free-choice in a cafeteria feeding system, and also the effect of restricting concentrates offered.  The roughages were selected to represent different grazing conditions prevailing in arid rangelands.  Eighteen growing she-camels were randomly allotted to three groups. Each group was assigned one of the three roughages offered ad lib for the duration of the whole experiment.  Results indicated that type of roughage and concentrate levels, and their interaction, affected (P<0.05) FIC and diet selection, and consequently live weight gain.  Average total and roughage DMI were 78.9 and 16.1, 83.9 and 22.5, 96.4 and 33.4 g DM/day/Kg0.75 for straw, hay and atriplex groups, respectively.  Irrespective of the roughage fed, growing camels consumed three-times as much corn grains as that from the cottonseed meal.  Limiting concentrates offered to 75% or 50% of ad lib intake, decreased FIC, while the proportion of roughages in DMI increased significantly, total OMI and total protein decreased and crude fibres intake increased, more so in the straw fed camels. The Atriplex fed camels recorded the higher ADG, followed by the hay fed ones and the straw fed mates grew the least, 516, 429 and 240 g/d, respectively. Restricting the level of concentrates offered decreased significantly (P<0.05) the ADG (691, 305 and 189 g/d in camels fed 100, 75 and 50% of ad lib concentrate intake. These results tend to indicate that growing camels having free choice to select their diets from both concentrates and roughages were capable of regulating their voluntary food intake predominantly through physiological mechanisms to satisfy energy requirements. This was true for the atriplex and hay groups but not for the straw group or when concentrates offered was limited.  [Journal of American Science. 2010;6(11):317-326]. (ISSN: 1545-1003).

Key words: camels, diet selection, feed intake capacity, weight gain

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Application of Proposed Distribution Network Planning Rules on Fast Developing Countries

Salem M. Elkhodary1, and M. Khafagy2

1 Faculty of Engineering, Ain Shams University,, Cairo, Egypt,2 Saudi Electricity Company, KSA.

Abstract: With the ever increasing need to electric energy and the fast development of loads in many countries especially in the fast developing ones such as the GULF countries, the load growth as well as the forecasted loads, are highly increased depending on new and arising factors and conditions. In turn, Electricity Companies build rapidly generating plants, transmission and distribution networks to meet the rapid load demand. Usually, power system expansion follows the load growth which may exist at random locations. This adds to the absence of prior proper planning, especially medium and long term planning, resulting in network configurations that do not match with optimum siting and sizing planning rules. Operation of such networks faces several problems that may sacrifice the power quality. Thus, proper planning of new networks, expansion or rehabilitation of existing ones should be based on most accurate and proper planning rules. This calls for the investigation of a new exact cost function for optimum sizing and siting of network substations, and hence the H.V. feeds (incoming) and the M.V. distribution (outgoing) feeders. Therefore, this paper presents a newly proposed methodology that takes into consideration the capital costs of all electrical components, losses in these components, operation and maintenance costs. The inflation rate can be also taken into consideration. This methodology gives important results, which conclude that the optimum distance between substations and hence the optimum number of substations, greatly depends on different factors that were not taken into consideration before, for example : the kWh price, cost of the HV incoming feeders (66-110 kV feeders) besides the cost of the MV outgoing feeders (6.6-22 kV feeders), cost of the distribution substations (MV/LV), cost of losses in transformers, cost of losses in all feeders, incoming and outgoing, Operation and maintenance costs ….etc. [Journal of American Science. 2010;6(11):327-]. (ISSN: 1545-1003).

Keywords: Cost function. Objective function. , Distribution network planning. Optimum siting and sizing of substation

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New Proposed Method of Damping Temporary Overvoltages on Power System Interconnections

Salem M. Elkhodary1, and Ali S. Abd El-Munem2

1 Faculty of Engineering,  Ain Shams University , 2 Egyptian Electricity Holding Company, Cairo, Egypt

Abstract: The interconnection between countries links different networks. These interconnections may be exposed to several disturbances. These disturbances (such as transient and temporary overvoltages phenomena, faults …etc.) threaten the interconnection security and reliability. This paper presents actual field measurements of transient and temporary overvoltages appearing on the Egyptian – Libyan system interconnection as an example. These overvoltages were recorded for different cases of operation. These cases were modeled and simulated using the most recent version of Alternative Transient Program (ATP) computer package to compare the results of the computational method with the actual field measurements. The comparison between the ATP output results and the actual field measurements were found less than ± 4%. Within the research activities of the Egyptian Electricity Holding Company (EEHC) temporary overvoltage phenomena on the Egyptian – Libyan interconnection network were detected. EEHC carried field measurements of the temporary overvoltage by using a special transient mobile test laboratory. This detected temporary overvoltage was due to the generated reactive power along the line on switching, in spite of this leading reactive power was compensated by connecting number of reactors at different nodes. The economical aspect has been taken into consideration to reduce the number of reactors to the network, which showed the best effect on damping the temporary overvoltage. This paper, thus, presents a proposed technique to damp the temporary overvoltage and keep the system voltage within the permissible limits by estimating the optimum number and location of reactors that must be connected to the network. [Journal of American Science. 2010;6(11):336-342]. (ISSN: 1545-1003).

Keywords: interconnection; networks; disturbances; Alternative Transient Program (ATP); Egyptian Electricity Holding Company (EEHC)

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Evaluation of Corynebacterium variabilis Sh42 as a degrader for different poly aromatic compounds

Yasser M. Moustafa1, Nour Sh. El-Gendy1, Salem A. Habib2, Sherif Ali1*

1Egyptian Petroleum Research Institute, Cairo, P.O. 11727, Egypt.

2Mansoura University, Faculty of Science, Damietta, Egypt.

Abstract: Corynebacterium variabilis sp. Sh42 is used to investigate the biodegradation potentials and metabolic pathways of different poly aromatic compounds (PACs) in batch flasks. Effects of PACs size, molecular weight, alkylation and their presence individually or in mixture on biodegradation potentials of Sh42 were studied; Naphthalene (Nap) as a model compound for di-aromatic ring; Antheracene (Ant) and Phenantherene (Phe) as model compounds for tri-aromatic ring; while Pyrene (Pyr) as a model compound for four-aromatic ring compounds were used as representatives for different PAHs. Dibenzothiophene (DBT), 4-methyldibenzothiophene (4-MDBT) and 4,6-dimethyldibenzothiophene (4,6-DMDBT) were taken as representative models for PASHs compounds. While, 2-hydroxybiphenyl (2-HBP) and 2, 2'-bihydroxybiphenyl (2, 2'-BHBP) were taken as models for phenolic compounds. The experimental results show that biodegradation rate decrease with increase ring size, alkylation’s group within homologous series and Sh42 has the highest capability to biodegradation of toxic phenolic compounds either in single  (BD% ≈ 90%) or mixed substrates cultures (BD% ≈ 48%). To ensure detoxification and mineralization of these toxic PACs; metabolic pathways of representative model compounds (Pyr, DBT and 2,2'-BHBP) were elucidated by GC/MS analysis which confirmed that, Sh42 completely metabolized all representative compounds to CO2 and H2O. [Journal of American Science. 2010;6(11):343-356]. (ISSN: 1545-1003).

Keywords: Polynuclear aromatic compounds, Biodegradation, Metabolic pathways.

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In vitro assessment of gastrointestinal viability of potentially probiotic Lactobacilli

Kawther,.EL-Shafei, N.F.Tawfik, Nadia, M.A.Dabiza, O.M.Sharaf,  and B.A.Effat  

Dairy Science Department, National Research Center Dokki, Cairo, Egypt.

Abstract: The objectives of this study were to assess the potential of four probiotic lactobacillus strains, Lactobacillus bulgaricus, Lactobacillus johnsonii B-2178, Lactobacillus gasseri B-14168 and Lactobacillus salivarius B-1950 in human upper gastrointestinal tract in vitro and evaluate the effect of milk proteins addition on viability of these strains in simulated gastric juices and in yoghurt during storage for 15 days at 4°C. The viability of lactobacilli strains in simulated gastric transit conditions (pH 2.0, pH 3.0 and pH 4.0) gastric juices with or without milk proteins singly or in combination with starch was tested. All the treatments were determined with three replicates. The simulated gastric transit tolerance of L.  johnsonii ,L. gasseri and L. salivarius strains was pH-dependent and correspondingly showed lower viability at pH 2.0 after 180 min compared with pH 3.0 and pH 4.0. The addition of milk proteins singly or in combination with starch enhanced the survival of probiotic lactobacilli strains in simulated gastric juices different tested pH values. Results showed that addition of milk proteins in combination with starch improved the viability of L.johnsonii B-2178, L. gasseri B-14168 and L. salivarius B-1950 in yoghurt during storage. Sensory evaluation showed that yoghurt fortified with milk proteins plus starch recorded the highest score for and overall acceptability than the other treatments. However, yoghurt manufactured with L. johnsonii and L. gasseri and fortified with sodium caseinate plus starch showed the highest organoleptic score. It is suggested that the yoghurt of acceptable quality and high total probiotic bacterial count during storage can be made from milk supplemented with 0.5% (w/v) starch plus 0.5% (w/v) sodium caseinate. [Journal of American Science. 2010;6(11):357-367. (ISSN: 1545-1003).

Keywords: Probiotics, Gastric tolerance, L. johnsonii, L. gasseri,L. salivarius

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Extraction of oil from canola seeds with supercritical carbon dioxide: Experimental and Modeling 

Soroush Zarinabadi1*, Riyaz Kharrat2, Ali Vaziri Yazdi3

1, 3-Islamic Azad University- Science & Research Branch – Tehran, Iran

2-Petroleum University of Technology - Tehran, Iran

1-zarinabadi@yahoo.com , 2-kharrat@put.ac.ir ,  3- avy123@behta.com

 ABSTRACT: In this work extraction oil from canola (Brassica Napus) seed  with supercritical CO2 extraction at pressure of 1500 to 2750 Psi , temperature of 308 to 333 k, and particles size 0.08 to 0.2 mm  in flow rate 5  Lit/hr was investigated in a bench scale apparatus, The extraction was modeled by the sovova extended lack’s model. The fluid phase mass transfer coefficient (kf), solid phase mass transfer coefficient (ks), and hardly accessible solute (xk) were a just able parameter of Models. The broken and intact cells model fit the experimental data, quite well, showing the applicability of the model to the supercritical extraction system studied here. [Journal of American Science. 2010;6(11):368-373. (ISSN: 1545-1003). 

Keywords: supercritical fluid extraction, canola oil, mathematical modeling, sovova model

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Biochemical Significance of Proinflammatory Cytokines in Psoriasis vulgaris among Egyptian Patients

 Halla M. Ragab*, Nabila Abd El Maksoud* and Mohamed M. Farid Roaiah** 

*Department of Biochemistry, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Cairo, Egypt. ** Dermatology & Andrology and S. T. D.S, Kasr El Aini Hospital, Cairo university. hmragab@yahoo.com 

Abstract: Background: Psoriasis has been characterized by hyperproliferation accompanied by acanthosis and aberrant differentiation of keratinocytes. Several growth factors and cytokines, are assumed to be important. Recent studies indicate that various cytokines including tumor necrosis factor - α ( TNF - α ), IL - 2R and IL - 6 play an essential role in the induction and maintenance of psoriatic lesion. Objectives: To analyse relevant inflammatory mediators in the serum of patients with active psoriasis ( Psoriasis vulgaris  ) of mild-to-moderate  and severe psoriasis  compared to healthy controls. Patients / Methods: Forty psoriasis patients were recruited from the dermatology outpatient clinic of Cairo University Hospital. Patients body mass index ( BMI ), waist circumference and psoriasis area and severity index.  (PASI ) were recorded. Fasting serum samples were obtained on enrolment. All the patients did not receive any treatment (locally or systemically), for at least four weeks before enrolment. Age, sex and ( BMI ) matched with forty healthy controls were also recruited. Serum TNF - α, IL - 2R and IL - 6 levels were estimated using an Enzyme-Linked Immunosorbant Assay ( ELISA ) technique. The patients group were subdivided to two groups according to the diseases severity, PASI , into, mild-to-moderate psoriasis group and severe psoriasis group. Results: Serum TNF - α, IL - 2R and IL - 6 were all statistically significant elevated in the patients group compared to healthy controls ( p < 0.05 ). Also they were all statistically significant increased in severe psoriasis compared to mild-to-moderate psoriasis ( p <0.05 ). Conclusions: These data support the view that serum TNF - α, IL - 2R and IL - 6 are involved in the pathogenesis of psoriasis, possibly by induction and maintenance of psoriatic lesion. We recommend a use of an array of these cytokines as a useful follow-up marker for monitoring of psoriatic patients and optimizing therapeutic strategies. Also we suggest the study of antisense therapy using the antibody of these cytokines in psoriatic patients. [Journal of American Science 2010;6(11):374-380]. (ISSN: 1545-1003).  

Keywords: Psoriasis vulgaris, Cytokines, TNF - α, IL - 2R and IL - 6

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Antihepatotoxic  Effect of Eruca Sativa Extracts on Alcohol Induced Liver Injury in Rats

Jihan Hussein1 , Azza Salah 2, Fatma Oraby 1, Amany Nour El-Deen  2 and Zakarya El-Khayat1 

1 Medical Biochemistry Department, National Research Center,Doki,Giza, 12311, Egypt

2 Biochemistry Department, Faculty of Science, Ain Shams University, 12311, Egypt

jihan_husein@yahoo.com

Abstract: Food derived antioxidants have a strong potential for long term use as chemopreventive agents in disease states involving oxidative stress, such as hepatitis and alcoholic liver diseases. This study aimed to investigate  the effect of different extracts of Eruca Sativa in ethanol induced liver injury in rats. Eighty eight male albino rats were divided into 3 main groups included control, prophylactic and treated groups using different extracts of Eruca sativa. Serum liver functions tests, lipid profile and oxidants/antioxidants profile were estimated. The results showed  that Eruca Sativa extracts improved liver functions, Lipid profile and antioxidants parameters. We concluded that, Eruca sativa extracts may exert their prophylactic and treatment role against oxidative stress produced by ethanol by increasing/maintaining the levels of antioxidant molecules and antioxidant enzymes. [Journal of American Science 2010;6(11):381-389]. (ISSN: 1545-1003). 

Key words: Eruca sativa, Ethanol, Liver, Ethanolic extract, Antioxidants, Oxidative stress

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In vivo and in vitro studies on Thevetia Species Growing in Egypt

I: Isolation, Identification, and Quantification of cardiac glycosides in in vivo and in vitro cultures of immature seeds.

Taha H. S.1*, Farag H.S.2, Shams A. K.2, Abdel-Azim S.N.2, Hanna G. A.3  Ewais E. E.4, Seif El-Nasr M. M.2

1 Plant Biotechnology Department, National Research Centre,  Dokki, Giza,  Egypt.

2Phytochemistry Department, National Research Centre,  Dokki, Giza,  Egypt.

3 Chemistry of Natural Compounds Department, National Research Centre,  Dokki, Giza,  Egypt.

4 Botany and Microbiology Department, Faculty of Science, Al-Azhar University ,Cairo, Egypt

Corresponding author hussein.taha2@yahoo.com

ABSTRACT: In vivo and in vitro extracted cardiac glycosides of immature seeds (IS) cultures of Thevetia neriifolia Jussieu. and T. thevetioides Kunth. were chemically identified. Calli were grown on modified Murashige & Skoog (MS) medium supplemented with 1mg/l 2,4-dichlorophenoxyacetic acid  (2,4-D) +3mg/l kinetin (Kin). The content of cardiac glycosides in IS cultures of T. neriifolia and T. thevetioides were monitored by HPLC. Two major compounds were detected and isolated from IS extracts i.e.  digitoxigenin and thevetin B. The different structures of the in vivo and in vitro isolated compounds were verified by means of MS and NMR spectral analysis, as well as those compounds were identified and determined using HPLC technique. [Journal of American Science 2010;6(11):390-395]. (ISSN: 1545-1003).

Key words: Cardiac glycosides, callus, Thevetia spp., HPLC, MS medium, immature seed cultures

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Comparative Analysis Of Resource Use Efficiency In Rice Production Systems In Abia State Of Nigeria

Nwaru, J. C. and O. R. Iheke

Department of Agricultural Economics

Michael Okpara University of Agriculture, Umudike

PMB 7267 Umuahia, Abia State, Nigeria

E- mail: nwaruj@yahoo.com

ABSTRACT: Arresting the observed low productivity and continued decline in the output of rice especially in the face of rising population and the concomitant escalating increases demand has been a lingering socioeconomic problem. Continued increase in rice production through a number of options including expansion into high potential areas especially the inland valleys has been proposed. This study was designed to examine resource use efficiency in rice production systems in Abia State of Nigeria. Primary data collected from a sample of 142 farmers consisting of 46 inland valley, 41 upland and 55 swamp rice farmers were analysed by the ordinary least squares multiple regression analysis and analysis of variance (ANOVA). Results indicate that the upland rice farmers are technically more efficient than the swamp and inland rice farmers and that there is no difference in technical efficiency between the swamp and inland rice farmers. None of the farmer groups achieved absolute allocative efficiency. The upland rice farmers achieved least allocative efficiency (Wij is farther from unity), underutilized all farm resources (Wij >1) while both the inland valley and the swamp rice farmers under utilized farmland, other inputs and capital and over utilised (Wij < 1) family labour and hired labour. There was no significant difference in the mean output of rice from the production systems; upland, inland valley and swamp while each operated in region one on the production surface indicating that overall, resource levels could be increased to achieve higher levels of productivity in each system. Economic policies and programmes that could encourage the reallocation and if possible the redistribution of farm production inputs for increased farm productivity and efficiency were recommended. [Journal of American Science 2010;6(11):396-408]. (ISSN: 1545-1003).

Key words: Resource use efficiency, rice production systems, Nigeria

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Knowledge Discovery In Al-Hadith Using Text Classification Algorithm

 Khitam Jbara

Jordan University, King Abdullah II School for Information Technology. ktjlc2000@yahoo.com

Abstract: Machine Learning and Data Mining are applied to language datasets in order to discover patterns for English and other European languages, but Arabic language belongs to the Semitic family of languages, which differs from European languages in syntax, semantic and morphology. One of the difficulties in Arabic language is that it has a complex morphological structure and orthographic variations. This study is conducted to examine knowledge discovery from AL-Hadith through classification algorithm in order to classify AL-Hadith to one of predefined classes (books), where AL-Hadith is the saying of Prophet Mohammed (Peace and blessings of Allah be upon him (PBUH)) and the second religious source for all Muslims, so because of its importance for Muslims all over the word knowledge discovery from AL-Hadith will make AL-Hadith more understandable for both muslims and nonmuslims. [Journal of American Science 2010;6(11):409-419]. (ISSN: 1545-1003).

Keywords: AL-Hadith, Classification, Stem, feature, Class, Expansion, Training set

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Organic amendment effect on soil properties and yield of potato (Solanum tuberosum) under irrigated condition: a case study from Kombolcha, Eastern Harergie, Ethiopia

Eyasu Mekkonen1, Fassil Kebede 2,3, * and Nurhussien Taha2

1Kombolcha Agricultural TVET College, Kombolcha, Eastern Harrargie; 2 Department of Land Resource Management and Environmental Protection, Mekelle University, Ethiopia

* Corresponding Author: Address: E-mail- fyimamu@gmail.com

Abstract: Field experiment was conducted in 2005/06 cropping season in Kombolcha to understand the comparative effect of organic and inorganic sources of soil ameliorant for managing surface soil crust under basin and furrow irrigation practices to boost potato production. A factorial experiment was conducted on plots of 12 m2 (4 m x 3 m) and arranged in RCBD with three replicates, which combine irrigation methods and soil amendments. The treatments were the control (no amendment), FYM, chat residue (decayed leaves of Chata edulis) and sediment (sub surface inorganic material locally known as ‘decay dimma’). Results have, therefore, revealed that FYM and chat made compost significantly (p < 0.05) improved moisture content, bulk density, porosity and infiltration rate over the sediment amended plot and the control. However, yield harvested from plots, which were amended with chat-made compost was significantly (p < 0.05) lower than FYM under furrow irrigation practice. [Journal of American Science 2010;6(11):420-425]. (ISSN: 1545-1003).

Keywords:  Chata edulis, decay dimma, organic amendment, potatoes, soil properties, irrigation

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The Effect of Women’s Socio-demographic Variables on their Empowerment

Hedayat Allah Nikkhah

Department of Social and Development Sciences, Faculty of Human Ecology, University Putra Malaysia, hnik2003@yahoo.com

Ma’rof Redzuan (Corresponding author)

Department of Social and Development Sciences, Faculty of Human Ecology, University Putra Malaysia. marof@putra.upm.edu,my

Asnarulkhadi Abu-Samah

Department of Social and Development Sciences, Faculty of Human Ecology, University Putra Malaysia. asnarul@putra.upm.edu.my

Abstract: Social scientists and development agencies have long been interested in the conditions that empower women. Since the empowerment could give women freedom of choice, equal access to domestic and community resources, opportunities and powers, thus, empowerment of women and the improvement of their status, particularly in respect of education, health and economic opportunities (occupation), are highly important ends in themselves. However, there are many factors which stop women benefiting from such development and contribute to women’s powerlessness such as inequality in economic opportunity, lack of knowledge, skills, and lack of access to education. This study elucidates the relationship between socio-demographic variable of women and their empowerment in Shiraz, Iran. Indeed, this study discovered the effect of respondents’ background i.e., age, education, occupation, family income and marital status on their empowerment. Data was collected from 195 women who participated in empowerment process which organized by NGOs in the whole city of Shiraz. The result of study showed that among the socio-demographic variables, only education and marital status had effect on empowerment. It can be concluded that access to education and knowledge play an important role increasing women empowerment. The result of study also showed that the divorced women have high level of empowerment compare to married and widow women. This is might due to that married women couldn’t get their husband permission to participate in empowerment process. 

[Hedayat Allah Nikkhah. The Effect of Women’s Socio-demographic Variables on their Empowerment. Journal of American Science 2010;6(11):426-434]. (ISSN: 1545-1003).

Key words: empowerment, socio-demographic variables, women, Iran

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Nutritive Aspects of Oxalis corniculata L.  Used by Tribals of Central India During Scarcity of Food.
 
Ashok k. Jain1, Preeti Tiwari Barua2 and Mudasir Bashir3

1Professor, School of Studies in Botany, Jiwaji University Gwalior -474011, Madhya Pradesh, India; E- mail: asokjain2003@yahoo.co.in

2Assistant Professor, Deptartment of Biotechnology, IPS Academy Indore-452012, Madhya Pradesh, India; E- mail: preetibarua26@gmail.com;

3Research Scholar, Plant Tissue Culture Laboratory, School of Studies in Botany, Jiwaji University Gwalior-474011, Madhya Pradesh, India; E-mail: mudasirbot@gmail.com

Abstract: Reports on ethnobotanical surveys reveal that a good number of plant species are being used by various tribal communities as emergency food. The present work deals with some parameters regarding nutritive value of leaves of Oxalis corniculata. L. used as alternative vegetable during emergency by some tribes of central India. The leaves have been found to be rich in moisture (82.42±0.5%), total carbohydrate (24.67±0.4%), crude protein (22.28± 0.5%), crude lipid (23.7±0.5%), sodium (1.12±0.02%), potassium (2.17± 0.31%), calcium (2.5± 0.08%), nitrogen (3.56±0.70 %) and magnesium (0.25±0.03%). [Ashok k. Jain, Preeti Tiwari Barua and Mudasir Bashir. Nutritive Aspects of Oxalis corniculata L.  Used by Tribals of Central India During Scarcity of Food. Journal of American Science 2010;6(11):435-437]. (ISSN: 1545-1003).

Key words:  Nutritive status;Oxalis cornicula; Tribes; Scarcity of food

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Effects of sports participation on psychological stress in female students in region 3 of Kermanshah

Ali Feyzkhademi1, Saadat Hajipoor1, Shahram Azimi2, Mehrdad Jalalian3, 4, 5

1Faculty Member of Izeh Branch, Islamic Azad University, Izeh, Iran

2Lecturer, Sama Branch (Kermanshah), Islamic Azad University, Kermanshah, Iran

3Research Center of Iranian Blood Transfusion Organization, Khorasan-e Razavi Blood Center, Mashhad, Iran

4Department of Community Health, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor D.E., Malaysia

5Editorial Office, Electronic Physician Journal, Mashhad, Iran

khademisport@gmail.com

Abstract: The aim of this study was to investigate the effects of sports participation on the psychological stress levels of female students 15-18 years old. Psychological stress is defined as a collection of nonspecific reactions against organisms in reflections and exposure to any factor that should be faced. Stress control includes several factors, and, in particular, sports participation is thought to be effective. This quasi-experimental research was performed using pre-test plan–test, after-test, and control groups. Research subjects for the control group were 30 people chosen randomly. The subjects of stress were tested by a 40-item stress questionnaire and then tested in step independent variable “Sports participation” included volleyball education and skills training for three months and three weekly sessions of 75-90 minutes. This was carried out to investigate its effect on the dependent variable "stress." We did not observe any statistically meaningful difference between the mean scores of stress-control group and experiment group scores at pre-test in p<0.05; however, statistically meaningful differences were observed between the mean scores of stress control group and experiment group scores on the post test stage (p<0.05) and between the mean scores of stress in the control group pre-test and post-test in (p<0.05). In addition, A statistically meaningful difference was observed statistically meaningful differences were observed between the mean scores of stress in the experiment group pre-test and post-test (p<0.05). [Journal of American Science 2010;6(11):438-441]. (ISSN: 1545-1003).

Keywords: Sports Participation; Psychological stress; Students

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[Journal of American Science 2010;6(11):442-448]. (ISSN: 1545-1003).

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Hepatoprotective and Therapeutic Activity of Origanum syriacum Aqueous Extract in Paracetmol Induced cell Damage in Albino Mice

Abeer Y. Ibrahim1, Nermeen M. Shaffie2 and Hemaia M. Motawa3

1Medicinal and Aromatic Plants Dept., Pharmaceutical and Drug Industries Division, National Research Centre.

2 Pathology Department, Medical researches Division, National Research Centre, Egypt.

3 ,Pharmacognosy Department, Pharmaceutical and Drug Industries Division, National Research Centre, 12622, Cairo, Egypt.

abeeryousry@yahoo.com

 Abstract: Ethnomedically genus Origanum L. is one of the most commonly used herb in many countries as a stimulant, analgesic, antitussive, expectorant, sedative, anti-inflammatory and antihelminthic agent. The hepatoprotective and therapeutic effects of Origanum syriacum aqueous methanolic extract on paracetamol  induced liver cell damage in mice with respect to antioxidant status was investigated. Mice were treated with extract and sylimarin in recommended dose after or before paracetamol administration (400mg/ kg/ day). Lipid peroxides concentration was considerably decreased due to the elevation of reduced glutathione concentration(GSH) and enhancing of glutathione reductase(GR), glutathione transferase(GST), glutathione peroxidase (GPx)and superoxide dismutase(SOD) activities as compared to paracetamol or sylimarin treated mice. Liver function parameters are still in the normal levels in extract treated mice as compared to control. Using extract as a treating agent after subjecting mice to paracetamol gave better results, the liver tissue showing a nearly normal liver tissue except for a little cellular infiltrate around main blood vessels while sylimarin showing a noticeable dilatation of blood vessels that are surrounded by fibrosis and cellular infiltration. Liver tissue from mouse received Origanum extract and then paracetamol showing mild dilatation of blood sinusoids and cellular infiltration around main blood vessels while sylimarin treated mice showed marked dilatation of blood sinusoids, vacuolar degeneration in many of the hepatocytes and focal necrotic areas among the hepatocytes. In conclusion, Origanum syriacum extract has potent therapeutic activity than hepatoprotective activity and it is more effective than sylimarin in two cases. The plant extract was screened for its phytochemical constitutions.

[Abeer Y. Ibrahim, Nermeen M. Shaffie and Hemaia M. Motawa. Hepatoprotective and Therapeutic Activity of Origanum syriacum Aqueous Extract in Paracetmol Induced cell Damage in Albino Mice. Journal of American Science 2010;6(11):449-458]. (ISSN: 1545-1003).

Key words: Hepatoprotective, Antioxidant, Oregano, Paracetamol, Therapeutic

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Water quality status of Golden Key Lake in Clement Town, Dehradun, Uttarakhand

Avnish Chauhan*, Mayank Pawar* and Showkat Ahmad Lone

* Department of Applied Sciences, College of Engineering, Teerthanker Mahaveer University, Moradabad-244001

Department of Environmental Science, Uttaranchal College of Science and Technology, Dehradun-248001

Abstract: An attempt has been made to understand to provide information on the physico-chemical characteristics of Golden Key Lake which is being used for aquaculture, were studied between Nov 2008 to Feb 2009. All the parameters has been correlated with each other and each parameters has shown correlation matrix with different parameters at selected sites.

[Avnish Chauhan, Mayank Pawar and Showkat Ahmad Lone. Water quality status of Golden Key Lake in Clement Town, Dehradun, Uttarakhand. Journal of American Science 2010;6(11):459-464]. (ISSN: 1545-1003).

Keywords: TDS, TSS, pH, DO, COD, Ca, Mg, K, Golden Key Lake

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Influence of freeze-shocked mesophilic lactic starter bacteria and adjunct lactobacilli on the rate of ripening Gouda cheese and flavor development

El-Sayed El-Tanboly, Mahmoud El-Hofi, Y. B. Youssef,*Wahed El-Desoki, and **Reda A. Jalil

Dairy Science Department, National Research Center, Dokki, Cairo, Egypt.

*Dairy Science Department, Al-Azhar Univ., Agriculture  Faculty, Assuet Branch, **Chamber of Food Industries, 1195 Cornish El-Nil, Beaulac, Cairo, Egypt.

tanboly1951@yahoo.com

Abstract: The objective of the present study was to determine the effects of Lactobacillus acidophilus on the sensory attributes, ripening time, and composition of Gouda cheese and to investigate the survival of L. acidophilus during ripening. Five types of Gouda cheeses, control cheese (Tc), made with with mesophilic lactic starter bacteria, Ta1, Ta2 , Tb1 and Tb2  cheeses made using modified mesophilic lactic starter bacteria by freezeshocked at -10oC/-20oC for  24 , 96 hrs  and probiotic Lactobacillus, as adjunct culture. Cheese samples were assessed for microbiological and compositional properties, proteolysis, and sensory evaluation at different ripening stages. The composition and the pH value were almost identical between control and experimental vats within a single trial cheese. Characterization of proteolysis of gel electrophoresis of cheese samples at various stages of ripening showed that the extent of casein degradation varied between samples in all cheeses, αS1-Casein was more extensively degraded than ß-casein. However, levels of soluble nitrogen (SN/TN) increased with ripening period for all cheeses, only moderate enhancement of proteolysis as in amino acid N in all trials. The formation of non protein nitrogen (NPN/TN) was slightly increased compared to control at the end of ripening. Organoleptic evaluation showed that probiotic cheese had higher sensory evaluation than control cheese, without probiotic strain. The population of Lactobacillus survived to numbers > 107 cfu/g, which is necessary for positive effects on health.  These results showed that the contribution of modified mesophilic lactic starter bacteria by freezeshocked and probiotic strain as adjunct culture can be successfully used in production of Gouda cheese.

[El-Sayed El-Tanboly, Mahmoud El-Hofi, Y. B. Youssef, Wahed El-Desoki, and Reda A. Jalil. Influence of freeze-shocked mesophilic lactic starter bacteria and adjunct lactobacilli on the rate of ripening Gouda cheese and flavor development. Journal of American Science 2010;6(11):465-471]. (ISSN: 1545-1003).

Keywords: Physically freeze-shock mesophilic starter, probiotic bacteria, proteolysis Gouda cheese

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Gap Analysis for Protected Areas of Andhra Pradesh, India for conserving biodiversity

C. Sudhakar Reddy

Forestry and Ecology Division, National Remote Sensing Centre, Indian Space Research Organisation, Balanagar, Hyderabad -500 625, India. drsudhakarreddy@gmail.com 

Abstract: A gap analysis was carried out to assess the Protected Area (PA) network system in Andhra Pradesh, India. The decisive factors of vegetation type distribution, elevation and endemism was used to determine the representativeness of PA system. In Andhra Pradesh, vegetation cover occupies 23.03% of geographical area and distributed in Coastal Plains, Deccan Plateau and Eastern Ghats. There are 27 PAs for conservation in Andhra Pradesh. The total area protected for biodiversity is about 12,555 km2 or 4.56% of geographical area of Andhra Pradesh. Of the three physiographic regions, Eastern Ghats represents very high area under PAs which was estimated as 7811.38 km2 followed by Deccan plateau of 3526.89 km2. Three main forest types (semi evergreen forests, thorn forests and dry evergreen forests) missing in the existing PA network were identified. Moist deciduous forests of Eastern Ghats of northern Andhra Pradesh were under-represented in PAs. The land area in an elevation range of 900m-1527m was  not included in PA network. Of the 103 species of endemics, 64 species were not included in PA system. Many PAs are experiencing threat from invasive species, forest fires, grazing pressure etc. There is a need to consider for possible ways for effective conservation and to extend the present PA network system in India.

[C. Sudhakar Reddy. Gap Analysis for Protected Areas of Andhra Pradesh, India for conserving biodiversity. Journal of American Science 2010;6(11):472-484]. (ISSN: 1545-1003).

Keywords: gap area; vegetation;  protected area; semi evergreen forests; Andhra Pradesh; India

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­Knowledge Discovery in Al-Hadith Using Text Classification Algorithm

Khitam Jbara

 Department of Computer Science, King Abdullah II School  for Information Technology, The University Of Jordan, P.O. Box 710481 Amman 11171 Jordan. ktjlc2000@yahoo.com

Abstract:  Machine Learning and Data Mining are applied to language datasets in order to discover patterns for English and other European languages, Arabic language belongs to the Semitic family of languages, which differs from European languages in syntax, semantic and morphology. One of the difficulties in Arabic language is that it has a complex morphological structure and orthographic variations. This study is conducted to examine knowledge discovery from AL-Hadith through classification algorithm in order to classify AL-Hadith to one of predefined classes (books), where AL-Hadith is the saying of Prophet Mohammed (Peace and blessings of Allah be upon him (PBUH)) and the second religious source for all Muslims, and because of its importance for Muslims all over the word knowledge discovery from AL-Hadith will make AL-Hadith more understandable for both Muslims and nonmuslims.

[Khitam Jbara. ­Knowledge Discovery in Al-Hadith Using Text Classification Algorithm. Journal of American Science 2010;6(11):485-494]. (ISSN: 1545-1003).

Keywords: AL-Hadith, classification, stem, feature, class, expansion, training set

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Efficacy of some Biocontrol Agents on Reproduction and Development of Meloidogyne incognita Infecting Tomato

Moussa Lobna* and Hanaa Zawam**

 *Soils, Water & Environment Research Institute, Agricultural Research Centre (ARC), Giza, Egypt,

** Plant Pathology Research Institute, Agricultural Research Centre (ARC), Giza, Egypt

mlobnamy@yahoo.com, hn_zawam@yahoo.com 

Abstract: Three rhizobacteria and two yeasts isolates were used as biocontrol agents against Meloidogyne incognita in laboratory and greenhouse. The used biocontrol agents were identified as Bacillus amyloliquefaciens, Brevibacterium otitidis, Sanguibacter inulinus, Candida incommunis and Wicherhamiella domercqiae. They inhibited the egg-masses hatching in vitro and exhibited strong nematicidal activity by killing the second stage juveniles of Meloidogyne incognita to various degrees in greenhouse. The most effective treatment was the complete culture of the four biocontrol agents (propagules and filterate) suppressed galls and egg-masses formation by 100% Br. otitidis reduced galls and egg-masses by 43.7 and 52.19 %, respectivily compared with the untreated control. The microorganisms used in greenhouse test reduced nematode populations in the rhizosphere and promoted the growth of tomato plants over the control treatment.

[Moussa Lobna and Hanaa Zawam. Efficacy of some Biocontrol Agents on Reproduction and Development of Meloidogyne incognita Infecting Tomato. Journal of American Science 2010;6(11):495-509]. (ISSN: 1545-1003).

Key words: Biocontrol; Meloidogyne incognita; tomato; Rhizobacteria; yeast

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Phytochemical and Elemental Analysis of Acalypha wilkesiana Leaf

*Madziga, H. A.1, Sanni S.2 and  Sandabe U. K.1

1Department of Veterinary Physiology, Pharmacology and Biochemistry, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria. 2Department of Veterinary Pharmacology, University of Abuja, Nigeria.

hannamadziga@yahoo.com

ABSTRACT: Phytochemical and Elemental determination of Acalyphy wilkesiana was conducted. The result of the Phytochemical analysis of the aqueous leaf extract of A. wilkesiana revealed a high presence of carbohydrates, Tannins and Flavonoid, a moderate  presence of Phlobatannins, Saponins. Alkaloids and Cardiac glycosides and minute quanity of Terpenes and Steroids. Anthraquinone derivatives was not present. The Elemental analysis showed presence of chloride, sodium, potassium, calcium, iron, magnesium, zinc copper and mangenese  in moderate quantity while cadmium and lead were not detected. It is therefore concluded that the aqueous leaf extract of A. wilkesiana contains Pharmacologically useful active principles elements. Thus the aqueous leaf extract of the plant could play a vital roles in health and disease.

[Madziga, H. A., Sanni S. and Sandabe U. K. Phytochemical and Elemental Analysis of Acalypha wilkesiana Leaf. Journal of American Science 2010;6(11):510-514]. (ISSN: 1545-1003).

Key words: Acalyphy wilkesiana, aqueous leaf extract , Phytochemical analysis, Elemental analysis

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Nutritional studies on some different sources of iodine on productive performance, ruminal fermentation and blood constituents of Buffalo. 1 – Effect of two different iodine levels on productive and reproductive performance of buffalo cows.

Kh. I. I. Zeedan1, O. M. El-Malky2, Kh. M. M. Mousa1 , A. A. El.Giziry1  and K. E.I. Etman1

1-     Department of Animal Nutrition Research.

2-     Department of Buffalo Research.

Animal Production Research Institute, Agricultural Research Centre, Dokki, Giza, Egypt.

khzeedan@yahoo.com

Abstract: This study was conducted to evaluate the effect of feeding buffalo cows on ration supplemented with two levels from iodine (I) during late pregnancy (three months before parturition) and postpartum period (six months after parturition) on nutrients digestibility, some blood constituent, birth weight of their offspring, Concentrations of immunoglobulin in colostrums, milk (yield and composition) and reproductive parameters. Eighteen buffalo cows (2-4 lactations) in late pregnancy period were selected to carry out the experimental work. The animals were divided into three similar groups (6 female buffaloes in each). Concentrate feed mixture (CFM), berseem hay (BH) and rice straw (RS) were given to animals as a control ration (I0) without supplementation, while the other groups I1 and I2 received the control ration with iodine at levels of 0.3 and 0.5  mg I per kg DM intake /h/d, respectively. Results indicated that supplementation ration of buffalo cows with different levels of I had improved the digestibility of all nutrients, TDN, DCP at pre and post partum, feed efficiency, increased milk yield, 7% fat correct milk yield and its composition. Birth and weaning weight of calves in treated groups were higher than that control group. Immunoglobulin concentration in colostrums indicated higher values with animals